Multi - color Flow Cytometry Mini Panel for T Cell Identification

10 Jun 2025 by AntBio
Multi - color Flow Cytometry Mini Panel for T Cell Identification
The plasticity of T cells enables them to adapt to different microenvironments and precisely regulate immune defense, such as anti - infection and anti - tumor responses. CD3 marks all T cells and is used to evaluate the total T - cell count. CD4 identifies helper/regulatory T cells and is essential for monitoring immune function. CD8 identifies cytotoxic T cells and reflects the ability to fight viruses and tumors.Detecting CD3, CD4, and CD8 allows for the rapid identification and quantification of T - cell subsets. This provides insights into immune function, assists in disease diagnosis, guides treatment decisions, evaluates the efficacy of immunotherapy, and monitors immune changes during treatment. CD45, a common antigen on white blood cells, is crucial for distinguishing white blood cells from non - white blood cells in non - immune tissue samples and for determining the proportion of target cells among total immune cells.

Human Panel Recommendations


Marker Fluorochrome Max EX/EM (nm) Corresponding Laser Filter Product Number Format (1 T represents one test)
CD3 Alexa Fluor® 488 494/517 Blue Laser 530/30 S0B1636 20/100/500 T available
CD4 PE 496, 566/576 Blue or Yellow - Green Laser 575/26 S0B1530 20/50/100/500 T available
CD8 Alexa Fluor® 647 653/669 Red Laser 660/20 S0B1551 20/50/100/500 T available
CD45 APC - Cy7 659/782 Red Laser 780/60 S0B1583 20/50/100/500 T available

Mouse Panel Recommendations


Marker Fluorochrome Max EX/EM (nm) Corresponding Laser Filter Product Number Format (1 T represents one test)
CD3 FITC 494/518 Blue Laser 530/30 S0B1783 20/100/500 T available
CD4 PerCP - Cy5.5 482/676 Blue Laser 695/40 S0B5133 20/50/100/500 T available
CD8 APC 651/660 Red Laser 660/20 S0B5086 20/50/100/500 T available
CD45 APC - Cy7 659/782 Red Laser 780/60 S0B1692 20/50/100/500 T available
Notes:
CD45 detection is essential for distinguishing between white blood cells and non - white blood cells in both human and mouse samples. It is required when analyzing non - immune tissue samples and when determining the proportion of target cells among total immune cells.

Detailed Explanation of Each Antibody Marker's Function:

CD antigen

Cellular expression

Molecular weight (kDa)

Functions

CD45

All hematopoietic cells

180–240 (multiple isoforms)

Tyrosine phosphatase, augments signaling through antigen receptor of B and T cells, multiple isoforms result from alternative splicing (see below)

CD3

Thymocytes, T cells

γ:25–28, δ:20, ε:20

Associated with the T-cell antigen receptor (TCR). Required for cellsurface expression of and signal transduction by the TCR

CD4

Thymocyte subsets, T H 1 and T H 2 T cells (about two thirds of peripheral T cells), monocytes, macrophages

55

Co-receptor for MHC class II molecules. Binds Lck on cytoplasmic face of membrane. Receptor for HIV-1 and HIV-2 gp120

CD8

Thymocytes subsets, cytotoxic T cells (about one third of peripheral T cells)

α: 32–34, β:32–34

Co-receptor for MHC class I molecules. Binds Lck on cytoplasmic face of membrane
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