Standard curve
Example of Mouse C3 standard curve in Assay Diluent #1.
Mouse & Rat C3 competitive OneStep ELISA Kit
Mouse & Rat C3 competitive OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | C3 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 6.1%； Inter-assay: 10.8% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Competitive |
| Sensitivity | 0.004 ug/mL |
| Range | 0.031 ug/mL – 2 ug/mL |
| Recovery | Cell culture supernatant: 113% Serum: 108% Plasma: 102% |
| Assay time | 60 minutes |
| Species reactivity | Ms;Rt |
Background
Mouse C3 is a central and essential component of the complement system, acting as the pivotal convergence point for all three complement activation pathways: classical, lectin, and alternative. This ~186 kDa protein, primarily synthesized in the liver, is proteolytically cleaved into two key fragments upon activation: the anaphylatoxin C3a, which recruits and activates immune cells, and the opsonin C3b, which tags pathogens for phagocytosis and amplifies the complement cascade. Its critical roles include mediating innate immune defense against infections, clearing immune complexes and apoptotic cells, and bridging innate and adaptive immunity. Consequently, Mouse C3 is a major focus of research in infectious disease, autoimmunity (e.g., lupus), neuroinflammation, and ischemia-reperfusion injury, with genetically modified C3-deficient mice being a fundamental tool for elucidating its precise functions in vivo.
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ELISA
Linearity
The concentrations of Mouse C3 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: C57 Mouse serum was 200fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 255.81 ug/mL in C57 Mouse serum.
#2 sample is undiluted samples are as follows: C57 Mouse plasma -Heparin was 200fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 262.95 ug/mL in C57 Mouse plasma -Heparin.
Linearity
#1 sample is undiluted samples are as follows: Lewis Rat serum was 200fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 185.22 ug/mL in Lewis Rat serum.
#2 sample is undiluted samples are as follows: Lewis Rat plasma -Heparin was 200fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 119.73 ug/mL in Lewis Rat plasma -Heparin.
#3 sample is undiluted samples are as follows: Lewis Rat plasma -Citrate was 200fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 173.53 ug/mL in Lewis Rat plasma -Citrate.
Protocol Diagram
