DNA Pull Down Kit(Plant)

Catalog Number: abs50073

Price:

$180.00 USD

Size: 6T

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Synonym

DNA pull down kit

Description

I. Experimental principle

DNA pull down technique is a powerful tool for studying DNA-protein interaction in vitro. In this technique, specific DNA probes are designed for the target region and labeled with desulfuryl biotin. After labeling, the labeled probes can bind to the streptavidin conjugated to the magnetic beads, and then incubated with the total protein extract. The non-specific binding proteins were removed by washing, and then the target DNA probe-protein complexes were obtained by elution. Finally, the protein types were identified by Western Blot or mass spectrometry (MS). The schematic diagram is as follows:

2, experiment flow chart

3, the flow chart of control group Settings

Composition

Component	Size	Storage
Nucleic-Acid Compatible Streptavidin Magnetic Beads	270uL	4°C
RNA binding buffer	6mL	4°C
Wash Buffer I	20mL	4°C
Protein-RNA binding buffer (10×)	600uL	4°C
IP lysis buffer	9mL	4°C
Protease inhibitor(100×)	35uL	-20°C
Glycerol (50%)	800uL	4°C
Wash Buffer II	10mL	4°C
Solution I	600uL	4°C
Elution Buffer	800uL	Avoid light at 4°C
DEPC H2O	400uL	4°C

Note:
1, need to bring your own Trizol, 75% ethanol, chloroform, isopropyl alcohol, reverse transcription kit and fluorescent dye;
2 and 6T were six single-group (one experimental group or one control group) immunoprecipitation experiments. In the later operation steps, one experimental group and one control group were included, and 2T reagent was needed.

General Notes

Suitable for plants

Storage Temp.

Store at -20 & 4 ° C (see component storage information for details); Valid for 1 year

Item added to your cart

DNA Pull Down Kit(Plant)