Standard curve
Example of cAMP standard curve in Assay Diluent #1R500.
cAMP OneStep competitive ELISA Kit
cAMP OneStep competitive ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | cAMP |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Others |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.6%； Inter-assay: 6.1% |
| Sample type | Cell culture supernatant, Serum, Plasma; lysates |
| Assay type | Competitive |
| Sensitivity | 0.199 pmoL/mL |
| Range | 7.59 pmoL/mL – 243 pmoL/mL |
| Recovery | Cell culture supernatant: 103% Serum: 110% Plasma: 102% |
| Assay time | 60 minutes |
| Species reactivity | Others |
Background
Cyclic adenosine monophosphate (cAMP) is a ubiquitous second messenger that plays a fundamental role in transducing extracellular signals into intracellular physiological responses. It is synthesized from ATP by adenylyl cyclase enzymes upon activation of G-protein-coupled receptors (GPCRs) and is degraded by phosphodiesterases (PDEs). The intracellular levels of cAMP directly regulate critical cellular processes, including metabolism, gene transcription, ion channel activity, cell proliferation, and apoptosis, primarily through the activation of protein kinase A (PKA) and the Exchange Protein directly Activated by cAMP (EPAC).
Picture
Picture
ELISA
Standard curve
Example of cAMP standard curve in Assay Diluent #1PR500.
Linearity
Linearity
The concentrations of cAMP were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Human serum was 5fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 824.62 pmoL/mL in human serum.
#2 sample is undiluted samples are as follows: Human plasma was 5fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1014.14 pmoL/mL in human plasma.
Linearity
The concentrations of cAMP were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: A549 lysates based on 1 mg/mL extract load(100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 104.98 pmoL/mL in A549 lysates.
Protocol Diagram
