Standard curve
Example of Human FSH standard curve.
Human FSH OneStep ELISA Kit
Human FSH OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | FSH |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 2.2%； Inter-assay: 3.1% |
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.066 pg/mL |
| Range | 0.20 pg/mL –12.48 mIU/mL |
| Recovery | Serum: 110% Plasma: 105% Cell culture supernatant: 115% |
| Assay time | 60 minutes |
| Species reactivity | Hu |
Background
Follicle-stimulating hormone (FSH) is a hormone your pituitary gland makes and releases that plays a role in sexual development and reproduction. It affects the function of ovaries and testicles. It is an important hormone that has a few different functions depending on the type of sexual organs (gonads) you have and your age. In males, FSH and LH work together to trigger their testes to begin producing testosterone. This is the hormone responsible for the physical changes of puberty (such as body hair growth and voice deepening) and the production of sperm. In females, FSH and LH trigger their ovaries to begin producing estrogen. This hormone is responsible for physical changes of puberty, like breast development and menstruation.
Picture
Picture
ELISA
Linearity
The concentrations of Human FSH were measured and interpolated from the target standard curves and corrected for sample dilution.
S#1 sample is undiluted samples are as follows: human serum (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 13.71 mIU/mL in human serum.
P#1 sample is undiluted samples are as follows: human plasma-heparin (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 4.62 mIU/mL in human plasma-heparin.
P#2 sample is undiluted samples are as follows: human plasma-EDTA (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 6.34 mIU/mL in human plasma-EDTA.
To assess the linearity of the assay, three samples were spiked with high concentrations of Human FSH in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, three samples were spiked with high concentrations of Human FSH in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Protocol Diagram
