IL-6 Protein, Human (GMP)
IL-6 Protein, Human (GMP)
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | IL-6, B-Cell Stimulatory Factor 2, BSF-2, HSF, IFNB2, Interleukin-6, CTL Differentiation Factor, CDF |
| Accession | P05231 |
| Amino Acid Sequence | Val30-Met212 |
| Expression System | E.coli |
| Mycoplasma | 阴性 |
| Sterility | 符合 |
| phValue | 7.7~8.3 |
| ProteinContent | 90%~120% |
| SdsPageMolecularWeight | 21 kD（还原） |
| SdsPagePurity | >97% |
| SecHplcPurity | >97% |
| CellActivity | < 0.2ng/ml |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 100mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1、Heinrich P C. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20. |
Background
Interleukin-6 (IL-6) is a member of the pro-inflammatory cytokine family, induces the expression of a variety of proteins responsible for acute inflammation, and plays an important role in the proliferation and differentiation of cells in humans. Interleukin 6 was originally identified as a T cell-derived lymphokine that induces final maturation of B cells into antibody-producing cells. Recombinant human IL-6 acts on B cells activated with Staphylococcus aureus Cowan I or pokeweed mitogen (PWM) to induce immunoglobulin M (IgM), IgG and IgA production, but not on resting B cells. Anti-IL-6 antibody was found to inhibit PWM-induced Ig production, indicating that IL-6 is one of the essential factors in PWM-induced Ig production. Furthermore, IL-6 was shown to augment the primary and secondary anti-SRBC (sheep red blood cell) antibody production in mice in vivo.
