Multi - color Flow Cytometry Mini Panel for Th1/2/17 Cell Subsets

08 Jun 2025 by AntBio
Multi - color Flow Cytometry Mini Panel for Th1/2/17 Cell Subsets
Th helper (Th) cells, CD4⁺ T - cell functional subsets, are categorized into Th1, Th2, and Th17 based on cytokine secretion and immune regulation. They play a central role in infection defense, allergy, autoimmune diseases, and tumor immunity.
Th1 cells : Key cytokine - IFN - γ. They activate macrophages and CD8⁺ T cells to eliminate intracellular pathogens (e.g., tuberculosis bacteria, viruses) and contribute to antitumor immunity by promoting cytotoxic responses.
Th2 cells: Key cytokine - IL - 4. They combat parasitic infections (e.g., helminths), activate eosinophils and mast cells, and drive B - cell antibody class switching (e.g., IgE production).
Th17 cells: Key cytokines - IL - 17A/F. They defend against extracellular bacteria and fungi (e.g., Candida albicans, Klebsiella pneumoniae) and participate in mucosal barrier immunity (e.g., in the gut and skin).
These subsets form a dynamic balance network through cytokine cross - talk, transcription factor inhibition, and immune equilibrium regulation, collectively coordinating immune responses.

Human Panel Recommendations

Marker Fluorochrome Max EX/EM (nm) Corresponding Laser Filter Product Number Format (1 T represents one test)
CD4 PE - Cy7 496, 566/576 Blue or Yellow - Green Laser 780/60 S0B1630 20/50/100 T available
IFN - γ FITC 494/518 Blue Laser 530/30 abs1840791 25/100 T available
IL - 4 APC 651/660 Red Laser 660/20 abs1850190 25/100 T available
IL - 17A PE 496, 566/576 Blue or Yellow - Green Laser 575/26 abs1840977 25/100 T available

Mouse Panel Recommendations

Marker Fluorochrome Max EX/EM (nm) Corresponding Laser Filter Product Number Format (1 T represents one test)
IFN - γ FITC 494/518 Blue Laser 530/30 abs1850475 25/100 T available
IL - 17A PE 496, 566/576 Blue or Yellow - Green Laser 575/26 561020 559502 25 μg 100 μg
IL - 4 APC 651/660 Red Laser 660/20 abs1850191 25/100 T available
CD4 PerCP - Cy5.5 482/676 Blue Laser 695/40 S0B5133
Notes:
If you need to know not only the proportion of Th1/2/17 among CD4⁺ cells but also their proportion among total T cells, add CD3 detection.
To detect cytokines, briefly stimulate samples in vitro. This activates cells to secrete cytokines and blocks cytokine secretion, allowing intracellular detection. Common reagents include:

Purpose Product Number Product Name Format
T-cell factor stimulation cocktail with inhibitor abs9935-100T Stimulation Cocktail Kit, With Brefeldin A 100 T
Transport protein inhibitor Brefeldin A abs810012 Brefeldin A 10/25/50 mg available
Cytokine stimulant PMA abs9107 PMA (Phorbol 12-Myristate 13-Acetate) 1/5/25 mg available
Cytokine stimulant Ionomycin abs9108 Ionomycin 1/5 mg available
Cytokine stimulant PMA + Ionomycin abs9934-100T Stimulation Cocktail Kit, Without GolgiPlug 100 T
Human T-cell stimulation and expansion reagent abs160019-1ml Anti-human CD3/CD28 monoclonal antibody beads 1ml
abs160014-500ug Humanized Anti-human CD3 Mab 500ug
abs160015-500ug Humanized Anti-human CD28 Mab 500ug
UA090033 CellXViva Human T cell Activation Kit S/L format
S0B0009 NA/LE Mouse anti-human CD3 mAb 1/5/25/50 mg available
S0B0010 NA/LE Mouse anti-human CD28 mAb 1/5/25/50 mg available
Mouse T-cell stimulation and expansion reagent UA090032 CellXViva Mouse T cell Activation Kit S/L format
S0B0661 Invivo anti-mouse CD3ε Recombinant mAb 1/5/25/50/100 mg available
S0B0003E NA/LE Syrian Hamster anti-mouse CD28 mAb 1/5/25/50 mg available

 

  1. IFN- , IL-4, and IL-17A detection requires cell membrane permeabilization. Commonly used reagents are as follows:
Purpose Product Number Product Name Format
Fixative abs9110 Fixation Buffer 50/100 ml available
Permeabilization Reagent abs9111-150mL Permeabilization Wash Buffer 150 ml

 

Detailed Explanation of Each Antibody Marker's Function:

 

CD antigen

Cellular expression

Molecular weight (kDa)

Functions

CD4

Thymocyte subsets, T H 1 and T H 2 T cells (about two thirds of peripheral T cells), monocytes, macrophages

55

Co-receptor for MHC class II molecules. Binds Lck on cytoplasmic face of membrane. Receptor for HIV-1 and HIV-2 gp120

IFN-γ

Th1 cells, NK cells, CD8 ⁺ T cells

~25 kDa (dimer)

Promoting inflammation and anti infection: activate macrophages to kill intracellular pathogens, enhance MHC expression and promote antigen presentation, inhibit Th2/Th17 differentiation

IL-4

Th2 cells, eosinophils, mast cells

~15-20 kDa (monomer)

Immune regulation and allergies: promote B cell secretion of IgE, induce Th2 differentiation, inhibit Th1/Th17 response

IL-17

Th17 cells, gamma delta T cells, innate lymphocytes (ILC3)

~35-45 kDa (IL-17A homodimer)

Mucosal defense and inflammation: recruit neutrophils, enhance epithelial barrier function, promote autoimmune pathology
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