Multi - color Flow Cytometry Mini Panel for TREGs

08 Jun 2025 by AntBio
Multi - color Flow Cytometry Mini Panel for TREGs
Tregs are a subset of CD4⁺ T cells with immunosuppressive functions. Their main role is to maintain immune tolerance, preventing autoimmune reactions and excessive inflammation. Tregs inhibit immune responses through various mechanisms, including cell - contact dependence, secretion of inhibitory cytokines, and metabolic interference. Treg research offers key insights into immune balance and has significant translational value in autoimmunity, oncology, infection, and transplantation.

Human Panel Recommendation 1


Marker Fluorochrome Max EX/EM (nm) Corresponding Laser Filter Product Number Format (1 T represents one test)
CD3 Alexa Fluor® 488 494/517 Blue Laser 530/30 S0B1636 20/100/500 T available
CD4 PerCP - Cy5.5 482/676 Blue Laser 695/40 S0B1606 20/50/100/500 T available
CD25 PE 496, 566/576 Blue or Yellow - Green Laser 575/26 abs1840310 25/100 T available
FOXP3 Alexa Fluor® 647 653/669 Red Laser 660/20 abs1840741 25/100 T available

Human Panel Recommendation 2

In human samples, CD25⁺CD127⁻ can replace CD25⁺FOXP3⁺ to identify Tregs, as FOXP3 detection requires nuclear membrane permeabilization.

Marker Fluorochrome Max EX/EM (nm) Corresponding Laser Filter Product Number Format (1 T represents one test)
CD3 Alexa Fluor® 488 494/517 Blue Laser 530/30 S0B1636 20/100/500 T available
CD4 PerCP - Cy5.5 482/676 Blue Laser 695/40 S0B1606 20/50/100/500 T available
CD25 PE 496, 566/576 Blue or Yellow - Green Laser 575/26 abs1840310 25/100 T available
CD127 APC 651/660 Red Laser 660/20 abs1840105 25/100 T available

Mouse Panel Recommendation


Marker Fluorochrome Max EX/EM (nm) Corresponding Laser Filter Product Number Format (1 T represents one test)
CD3 FITC 494/518 Blue Laser 530/30 S0B1783 20/100/500 T available
CD4 PerCP - Cy5.5 482/676 Blue Laser 695/40 S0B5133 20/50/100/500 T available
CD25 Alexa Fluor® 647 653/669 Red Laser 660/20 S0B1545 20/50/100/500 T available
FOXP3 PE 496, 566/576 Blue or Yellow - Green Laser 575/26 abs182408 25/100 T available

Reagents for FOXP3 Detection

FOXP3 detection requires nuclear membrane permeabilization. Commonly used reagents include:

Purpose Product Number Product Name Format
Fixative abs9110 Fixation Buffer 50/100 ml available
Nuclear Permeabilization Buffer (10×) abs90064 Permeabilization Buffer 100 ml

Detailed Explanation of Each Antibody Marker's Function:

CD antigen

Cellular expression

Molecular weight (kDa)

Functions

CD3

Thymocytes, T cells

γ:25–28, δ:20, ε:20

Associated with the T-cell antigen receptor (TCR). Required for cellsurface expression of and signal transduction by the TCR

CD4

Thymocyte subsets, T H 1 and T H 2 T cells (about two thirds of peripheral T cells), monocytes, macrophages

55

Co-receptor for MHC class II molecules. Binds Lck on cytoplasmic face of membrane. Receptor for HIV-1 and HIV-2 gp120

CD25

Activated T cells, B cells, and monocytes

55

IL-2 receptor α chain

FOXP3

Treg，transiently expressed in activated conventional CD4+T cells (but has no inhibitory function)，some tumor cellsmay abnormally express FOXP3, but its function is still unclear (controversial)

47-50

It is a core regulatory factor for Treg development and function

CD127

Bone marrow lymphoid precursors, pro-B cells, mature T cells, monocytes

68–79, possibly forms homodimers

IL-7 receptor

 

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