ANT Bio Solution - 3D Organoid Culture

3D cell culture, which refers to growing cells in a three-dimensional culture environment, is a popular method in cell culture in recent years. With the emergence of 3D culture, cell culture models are closer to the in vivo physiological environment and show a high consistency with animal models in terms of drug test results. This can reduce reliance on animal experiments and shorten the research and development cycle, making it bound to become the trend in cell culture. Organoids – 3D cell cluster structures similar to organs with certain morphological structures and functions, formed by culturing embryonic or adult stem cells in vitro to allow them to proliferate and differentiate. Although these cellular structures are not real human organs in the true sense, they can simulate real organs in structure and function, and therefore, they are playing an increasingly important role in scientific research.

1.2 Culture System of Organoids

20 May 2026 by ma kun

The core of in vitro 3D culture of organoids is to understand the composition of the in vivo stem cell microenvironment, and then simulate the in vivo microenvironment in the in vitro culture system to induce stem cell proliferation and differentiation to form specific organs or tissues.

(1) Cytokines: cell growth regulators (cytokines or certain small molecules) involved in maintaining stem cell self-renewal and proliferation and differentiation, such as EGF, Noggin and R-spondin etc.;

(2) Extracellular matrix: extracellular matrix that mimics the microenvironment in which stem cells grow. For example, cells are cultured in the three-dimensional space formed by matrigel, matrigel can replace the feeder layer cells in traditional culture systems, providing a three-dimensional environment for stem cell proliferation and differentiation, promoting cell aggregation in 3D culture and generating cell arrangement polarity.