Flow cytometry is a technique that uses a flow cytometer to perform individual, multi-parameter, rapid qualitative and quantitative analysis or sorting of single-file cells or biological particles in a state of rapid linear flow. It features fast detection speed, multiple measurement parameters, large data collection volume, comprehensive analysis information, high sorting purity, and flexible methods. It can be used for the detection of cell size, cell granularity, DNA and RNA content, protein content, cell-specific antigens, cell viability, intracellular pH value, cell cycle, cell apoptosis, and cell function analysis, among others. Since the 1970s, with the increasing level of flow cytometry, this technology has been widely applied in numerous scientific research and clinical medical fields such as cell biology, molecular biology, immunology, hematology, oncology, genetics, pharmacy, botany, and marine biology.