Flow cytometric analysis of FMC63 scfv Protein expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with FMC63 scfv Protein (right panel) at 0.1ug/test or unlabel (left panel). APC anti-His tag antibody was used as the secondary antibody. Flow cytometry and data analysis were perfommed using Agilent NovoCyte Quanteon and FlowJorM software.
FMC63 scFv Protein, His Tag
FMC63 scFv Protein, His Tag
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Product Details
Product Details
Product Specification
| Molecular Weight | Predicted MW: 27.6 kDaObserved MW: 27.6 kDa |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4 with 3% trehalose. |
| Reconstitution | Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation. |
| Stability & Storage | 12 months from date of receipt, -20 to -70 °C as supplied. |
Background
FMC63 scFv is a recombinant single-chain variable fragment constructed from the VH and VL domains of murine anti-human CD19 IgG2a monoclonal antibody FMC63 linked by a flexible peptide linker; it retains high specific binding affinity to human CD19 broadly expressed on normal B cells and B-cell malignant tumors, serves as the classic antigen-recognition extracellular domain for CD19-targeted CAR-T cells adopted in four FDA-approved CAR-T therapeutics including Kymriah and Yescarta, and is recombinantly manufactured for preclinical immunotherapy construction, CD19 binding verification, CAR detection and affinity characterization assays.
Picture
Picture
FC
SDS-PAGE
2μg（R: reducing conditions）
