2.2 Viability Dyes

Due to the overlapping of fluorescence spectra, when performing flow cytometry multicolor analysis, it is necessary to set up single-stained tubes for fluorescence compensation. The size of compensation beads is comparable to that of lymphocytes, and they can be conjugated with various fluorescently labeled antibodies to adjust compensation. Each drop of beads contains two populations: one is a positive population that can bind to antibodies, and the other is a negative population that does not react with antibodies.