Flow cytometric analysis of IL-2 expression in C57BL/6 mouse splenocytes stimulated with PMA and Ionomycin in the presence of Brefeldin A for 6hr (right Panel) or unstimulated (left Panel).
The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with SDT Alexa Fluor® 488 Rabbit Anti-Mouse IL-2 Antibody at 0.2 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Rabbit Anti-Mouse IL-2 Antibody (S-2117-6)
Alexa Fluor® 488 Rabbit Anti-Mouse IL-2 Antibody (S-2117-6)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | IL-2 |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P04351 |
| Clone Number | S-2117-6 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Isotype Control | S0B0161 |
| Application | ICFCM |
| Reactivity | Ms |
| Positive Sample | PMA stimulated C57BL/6 mouse splenocytes |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 0.2μg per million cells in 100μl volume | Ms |
Background
Interleukin-2 (IL-2) is a cytokine that plays a crucial role in the immune system. It is primarily produced by activated T cells and has multiple functions. IL-2 promotes the proliferation and differentiation of T cells, enhancing their ability to fight infections and tumors. It also stimulates the growth and activity of natural killer (NK) cells, which are important for immune defense. Additionally, IL-2 can regulate the activity of regulatory T cells (Tregs), helping to maintain immune tolerance and prevent autoimmune diseases. In clinical settings, IL-2 has been used to treat certain cancers by boosting the immune response, although its use is limited due to potential side effects. Its discovery and research have significantly advanced our understanding of the immune system and provided new strategies for immunotherapy.
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