1 0

    One-Step Rapid Mycoplasma Detection Kit (Isothermal Amplification)

    One-Step Rapid Mycoplasma Detection Kit (Isothermal Amplification)

    Catalog Number: abs90062 Brand: Absin
    Share:
    Price:
    $157 USD
    $157 USD
    Size:

    For shipping services or bulk orders, you may request a quotation.
    Secure checkout with
    View full details

    Product Details

    Product Specification

    Usage 1. Preparation before the experiment:
    65°C Water bath or metal bath

    2. Operation steps:
    1. Preparation of sample to be tested
    (1) Adherent cells: the supernatant was aspirated directly. It is recommended to inoculate or change fluid in cells 3 Days or more, the degree of confluence reaches 90% When sampling left and right, the mycoplasma content in the supernatant is high and easy to detect.
    (2) Suspension cells: 1000 rpm Centrifugation 5min After that, take the supernatant. It is recommended to inoculate or change fluid in cells 3 Samples are taken at more than days. At this time, the mycoplasma content in the cell culture medium is high and it is easy to detect.

    2. Reaction system,
    Component 25uL
    Mycored Buffer 23uL
    MycoRed Enzyme 1uL
    (1) Mycored Buffer After thawing, reverse and mix well for use.
    (2) According to the number of samples, after preparing the above reaction system , Dispense into a reaction tube and add 20 uL Of Paraffin Oil , and proceed to the next step.

    3. sample addition
    (1) Negative control: No sample is added to the first reaction tube as a negative control;
    (2) Positive control: add to the last reaction tube 1uL Positive Control As a positive control;
    (3) Sample to be tested: Add to the remaining reaction tubes 1uL Cell culture supernatant.
    Please reach the tip of the gun towards Paraffin Oil Add samples under the liquid level.

    4. Reaction conditions.
    Transfer the reaction tube to a pre-heated 65°C Incubate in a water pot or metal bath 30 min.

    3. Result judgment:
    Take out the reaction tube immediately after the end of the reaction, and wait until it returns to room temperature , If the reaction solution is still yellow, the judgment result is negative; If the reaction solution turns red, the judgment result is positive. The reaction tube cannot be opened , Otherwise, it will easily lead to aerosol pollution.
    Description This product uses isothermal amplification technology and visual color changes to quickly detect common cell culture fluid 8 Mycoplasma species, without requirement DNA Extract and add directly to the reaction solution 1 µL Cell culture supernatant, 65℃ reaction 30 min , the color of the positive sample reaction solution changes from yellow to red, and the whole process does not need to PCR The instrument does not need to open the lid for electrophoresis, which can effectively avoid cross-contamination caused by aerosols. With the traditional PCR Compared with the method, this product is simple to operate, has very high sensitivity, and can tolerate a variety of medium PCR Inhibitors, so there will be no false negative phenomenon, and the test results are the same as qPCR With extremely high consistency.

    Product composition:
    Product Code Product Name Specifications
    A Mycored Buffer 1.2mL
    B Mycored Enzyme 50uL
    C Positive Control 50uL
    D Paraffin Oil 1mL
    General Notes 1. Mix the Mycored Buffer well and use it.
    2. Do not open the reaction tube after the reaction. There are many isothermal amplification products, and factors such as aerosols can easily pollute the experimental environment
    3. It is recommended to use a suction tip with a filter element during the preparation of the reaction solution, and put the discarded suction tip and reaction tube into a ziplock bag for timely treatment.
    4. In order to ensure the reliability and stability of cell experiments, it is recommended to conduct regular mycoplasma contamination detection.
    5. For your safety and health, please wear a lab coat and disposable gloves.
    Storage Temp. Store at-20 ℃, protect from light, shelf life is 12 months.