Protein Purification Magnetic Beads/Kits - Efficient Capture of Target Molecules

16 Jun 2025 by AntBio

Experimental Principle

Protein purification magnetic beads/kits achieve precise capture and purification of target molecules based on specific biomolecular interactions. The surface of the magnetic beads is modified with high-affinity ligands (such as antibodies, streptavidin, His-tag binding groups, etc.), which can specifically bind to the target protein. For example, antibody-coupled magnetic beads selectively adsorb the target protein through the antigen-antibody binding principle, while streptavidin magnetic beads utilize the high affinity of biotin-avidin to capture biotinylated molecules.

After binding, magnetic separation technology is used to quickly separate the magnetic bead-target molecule complex from impurities, followed by washing to remove non-specific adsorption. Finally, the high-purity target product is released by changing the buffer conditions (such as pH adjustment, competitive elution). This technology has the advantages of high efficiency, gentleness, and automation, and is suitable for the purification of trace targets in complex samples (such as serum, cell lysates).

Experimental Procedure (Using Antibody-Conjugated Magnetic Beads as an Example)

1、Sample Pretreatment

Centrifugal Clarification: Centrifuge the sample (such as serum) at 3000g for 10 minutes to remove cellular debris or particulate matter.

Lysis (Optional): If the target is intracellular components, treat the sample with a lysis buffer containing protease inhibitors, sonicate to disrupt cells, and then centrifuge to collect the supernatant.

2、Magnetic Bead Incubation

Magnetic Bead Activation: Vortex mix the magnetic bead suspension, take an appropriate amount of magnetic beads into a centrifuge tube, and wash twice with pre-chilled PBS to remove the storage solution.

Binding Target Molecules: Mix the pretreated sample with the magnetic beads and incubate at room temperature with rotation for 30 minutes to ensure full contact.

3、Magnetic Separation and Washing

Magnetic Absorption to Remove Supernatant: Place the centrifuge tube on a magnetic rack and let it stand for 2-3 minutes until the solution is clear, then aspirate the supernatant.

Washing to Remove Impurities: Add washing buffer (such as PBS containing 0.1% Tween-20), gently resuspend the magnetic beads, and repeat the magnetic absorption step 2-3 times to remove non-specific bindings.

4、Target Molecule Elution

Competitive or Conditional Elution:

Acidic Elution: Add a low-pH buffer (such as glycine-HCl with pH 2.8), incubate for 5 minutes, neutralize immediately, and transfer the supernatant to protect the activity of the target.

Biotin Competition: For streptavidin magnetic beads, add an excess of free biotin to competitively release the target molecules.

Magnetic Bead Regeneration (Optional): Some kits support magnetic bead regeneration, which can be reused after treatment with deionized water and regeneration buffer.

Total Time: Approximately 60 minutes, with inter-batch variation <5%, and recovery rate can reach over 90%.

Product Advantages

Release microspheres to break free from the limitations of sample volume, reagent flow rate, column pressure, and elution volume.
Simultaneously achieve solid-liquid and liquid-liquid separation, simplifying steps, reducing loss, and saving time.
Break through the technical bottlenecks in the purification of secreted and inclusion body proteins.
Reduce dependence on instruments and equipment, lowering maintenance and service costs.
Stable operation, facilitating high-throughput and large-scale protein purification.
Stable product performance with minimal batch-to-batch variation.
Achieve high yield and high purity of the target protein.

Related Products

Item No.	Product Name	Applications and Features
abs9905	Protein G Agarose Beads	Efficient purification of IgG, suitable for various species, reduces non-specific binding
abs9904	Protein A Agarose Beads	Specific binding to IgG, suitable for human, rabbit, mouse, and other species, high binding capacity
abs90154	His-tag Protein Purification Beads (IDA-Ni)	Utilizes nickel ions for specific binding to His-tag proteins, suitable for large-scale purification
abs90155	His-tag Protein Purification Beads (IDA-Co)	Utilizes cobalt ions for specific binding to His-tag proteins, suitable for large-scale purification
abs90156	His-tag Protein Purification Beads (NTA-Ni)	Utilizes nickel ions for specific binding to His-tag proteins, suitable for small-scale purification
abs90157	His-tag Protein Purification Kit (IDA-Ni)	Includes nickel ion beads and reagents, suitable for large-scale His-tag protein purification
abs90158	His-tag Protein Purification Kit (IDA-Co)	Includes cobalt ion beads and reagents, suitable for large-scale His-tag protein purification
abs90166	Protein A/G Agarose Beads	Includes Protein A and G beads, suitable for antibody purification
abs90170	Protein A/G Agarose Beads	Includes Protein A/G beads and reagents, suitable for antibody purification
abs90146	Protein A/G Magnetic Beads	Combines Protein A and Protein G advantages, suitable for various IgG types
abs90159	GST Fusion Protein Purification Beads	Includes GST beads and reagents, suitable for GST fusion protein purification
abs90161	GST Fusion Protein Purification Kit	Includes GST beads and reagents, suitable for GST fusion protein purification
abs90162	Strep-tag II Protein Purification Beads	Specifically binds to Strep-tag II protein, suitable for protein purification and separation
abs90163	Strep-tag II Protein Purification Kit	Includes Strep-tag II beads and reagents, suitable for Strep-tag II protein purification
abs90164	Heparin Magnetic Beads	Utilizes heparin for specific binding to certain proteins, suitable for protein purification
abs90165	DEAE Magnetic Beads	Utilizes anion exchange groups for specific binding to negatively charged proteins, suitable for protein purification

Absin provides antibodies, proteins, ELISA kits, cell culture, detection kits, and other research reagents. If you have any product needs, please contact us.

Absin Bioscience Inc.
Email: worldwide@absin.net