PKC η recombinant rabbit monoclonal antibody: How to become a key tool for precise exploration of cell signaling?

I. Why Do Traditional Antibodies Face Limitations in PKC η Research?

Before the maturation of recombinant rabbit monoclonal antibody technology, researchers primarily relied on traditional polyclonal antibodies or murine monoclonal antibodies for PKC η studies. Both approaches have inherent drawbacks that limit the depth and accuracy of research.

Polyclonal antibodies are typically purified from the serum of immunized animals and consist of a mixture of antibodies targeting multiple epitopes. This characteristic leads to higher cross-reactivity. Among PKC family members, especially in the catalytic domain, there is high sequence homology. Therefore, when using polyclonal antibodies to detect PKC η, cross-reactivity with other PKC isoforms (such as PKC δ and PKC ε) is highly likely, resulting in nonspecific bands in Western Blot or high background and blurred localization in immunohistochemistry (IHC). This compromises the signal-to-noise ratio and specificity of experimental results.

Traditional murine monoclonal antibodies are produced via hybridoma technology. While they address batch-to-batch variability, their epitope diversity is limited by the murine immune system. For highly conserved proteins like PKC η, it may be challenging to screen for monoclonal antibodies that target unique variable regions with high affinity. Additionally, murine antibodies may exhibit insufficient sensitivity or nonspecific binding when applied to human tissue samples due to species differences. These limitations make it difficult to observe subtle changes in PKC η expression or subcellular localization in complex physiological and pathological processes.

II. How Does Recombinant Rabbit Monoclonal Antibody Technology Achieve Superior Performance?

The advent of recombinant rabbit monoclonal antibody technology marks a significant innovation in the field of antibody engineering. This technology cleverly combines the advantages of the rabbit immune system with recombinant DNA techniques such as phage display or single B-cell cloning, opening new pathways for producing high-performance research antibodies.

In principle, the technology first immunizes rabbits with highly purified PKC η antigens (such as recombinant proteins or peptides of specific functional domains). Rabbits possess a richer antibody gene repertoire and a stronger ability to generate high-affinity antibodies, especially for conserved proteins. Subsequently, B lymphocytes are isolated from the spleen or peripheral blood of immunized rabbits, and the variable region genes of their antibody light and heavy chains are obtained via molecular cloning. These genes are cloned into expression vectors and produced through recombinant expression in mammalian cells (e.g., HEK293 cells). This process entirely bypasses traditional hybridoma technology, achieving full controllability.

The core advantages are reflected in three aspects: First, exceptional specificity and affinity. Leveraging the rabbit immune system and recombinant technology, candidate antibodies undergo rigorous screening and validation, ensuring precise recognition of unique epitopes of PKC η and minimizing cross-reactivity with other PKC isoforms. Second, unparalleled batch consistency. Recombinant antibody production is based on fixed DNA sequences, ensuring identical performance across different production batches and long-term comparability and reproducibility of experimental results, which is crucial for long-term research projects. Finally, broad applicability and flexibility. Recombinant rabbit monoclonal antibodies perform excellently across multiple experimental platforms, including but not limited to Western Blot, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and immunohistochemistry. Their high affinity enables clear signal detection even in challenging samples like paraffin-embedded tissues.

III. In Which Cutting-Edge Research Areas Does PKC η Recombinant Rabbit Monoclonal Antibody Play a Key Role?

With its outstanding performance, PKC η recombinant rabbit monoclonal antibody has become an indispensable tool for in-depth exploration of the protein's biological functions, playing a critical role in multiple cutting-edge research areas.

In cancer biology research, PKC η has been found to exhibit abnormal expression in various epithelial-derived tumors, with context-dependent roles as either an oncogene or tumor suppressor. Using highly specific recombinant rabbit monoclonal antibodies, researchers can accurately assess PKC η expression levels and subcellular localization (e.g., cytoplasmic, nuclear, or membrane translocation) in tissue sections from different tumor stages and grades, correlating these findings with clinical-pathological features and patient prognosis. This provides a solid data foundation for exploring PKC η as a potential biomarker or therapeutic target.

In cell signaling network analysis, PKC η is a key node in multiple receptor downstream pathways (e.g., GPCR and RTK pathways). Through immunoprecipitation combined with mass spectrometry (IP-MS), this antibody can efficiently capture protein complexes interacting with PKC η, mapping dynamic interaction networks and revealing novel regulatory mechanisms in specific signaling pathways. Meanwhile, dual-label immunofluorescence experiments under confocal microscopy enable real-time observation of PKC η's spatiotemporal dynamics in cells under conditions such as growth factor stimulation, drug treatment, or stress, visually illustrating its activation and inactivation processes.

In neuroscience and immune regulation research, PKC η's functions in the central nervous system and specific immune cell subsets are gradually being uncovered. High-sensitivity recombinant antibodies make it possible to precisely quantify and localize PKC η in samples with limited material or low antigen content, such as brain tissue sections or primary immune cells, aiding in elucidating its roles in neural development, synaptic plasticity, and immune cell activation and differentiation.

IV. Which Manufacturers Provide PKC η Recombinant Rabbit Monoclonal Antibodies?

Hangzhou Start Biotech Co., Ltd. has independently developed the "PKC η Recombinant Rabbit Monoclonal Antibody" (Product Name: PKC η Recombinant Rabbit mAb (S-1053-35), a high-specificity, high-sensitivity, and highly stable novel tool for protein kinase C detection. This product is developed using recombinant rabbit monoclonal antibody technology and has been rigorously validated across multiple platforms, including Western Blot (WB), immunofluorescence (IF), and immunoprecipitation (IP). It holds significant research value in areas such as epithelial differentiation regulation, skin barrier function, and inflammatory responses.

Professional Technical Support: We provide comprehensive product technical documentation, including staining examples in epithelial tissues, co-localization studies with differentiation markers, and expert technical consultation, fully supporting customers in making progress in epithelial biology and immunology research.

Hangzhou Start Biotech Co., Ltd. is committed to providing high-quality, high-value biological reagents and solutions for global innovative pharmaceutical companies and research institutions. For more details about the "PKC η Recombinant Rabbit Monoclonal Antibody" or to request a sample test, please contact us.

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