MultiColor Multi - color Flow Cytometry Mini Panel for Macrophage Identification
11 Jun 2025
by AntBio
Macrophages, white blood cells residing in tissues, originate from monocytes, which in turn come from bone marrow precursor cells. Both macrophages and monocytes, as phagocytes, play a role in vertebrates' non - specific (innate immunity) and specific (cell - mediated immunity) defense mechanisms. Their primary function is to carry out phagocytosis (engulf and digest) of dead cells, cellular debris, and pathogens in a fixed or wandering cell form, and to activate lymphocytes or other immune cells, thereby speeding up the immune response to pathogens.
Panel Recommendations
| Marker | Fluorochrome | Max EX/EM (nm) | Corresponding Laser | Filter | Product Number | Format (1 T represents one test) |
| CD45 | APC - Cy7 | 659/782 | Red Laser | 780/60 | S0B1583 | 20/50/100/500 T available |
| CD68 | Alexa Fluor 647 | 653/669 | Red Laser | 660/20 | S0B5050 | 20/50/100/500 T available |
| CD86 | PE (either option) | 496, 566/576 | Blue or Yellow - Green Laser | 575/26 | S0B1673 | 20/50/100/500 T available |
| CD80 | PE (either option) | 496, 566/576 | Blue or Yellow - Green Laser | 575/26 | S0B1540 | 20/50/100/500 T available |
| CD206 | Alexa Fluor庐 488 | 494/517 | Blue Laser | 530/30 | S0B1823 | 20/50/100/500 T available |
Notes:
CD68 detection requires membrane permeabilization.
Neither CD11b nor CD14 can be used as a sole substitute for CD68, as they represent all myeloid cells and total monocytes, respectively. In conventional panels, they assist in cell clustering when co - stained with CD68.
For M2 macrophages, markers like CD163 can also be selected.
Detailed Explanation of Each Antibody Marker's Function:
| CD antigen | Cellular expression | Molecular weight (kDa) | Functions |
| CD45 | All hematopoietic cells | 180–240 (multiple isoforms) | Tyrosine phosphatase, augments signaling through antigen receptor of B and T cells, multiple isoforms result from alternative splicing (see below) |
| CD68 | Monocytes, macrophages, neutrophils, basophils, large lymphocytes | 110 | Lysosomal markers: involved in intracellular degradation and antigen processing. Phagocytosis: Identifying apoptotic cells and pathogen associated molecular patterns. Diagnostic application: markers of Macrophages in histopathology. |
| CD80 | Antigen presenting cells (APCs): | 60 | Co-stimulator, ligand for CD28 and CTLA-4 |
| Dendritic cells, macrophages, activated B cells. | |||
| Under inflammatory conditions, T cells and NK cells can induce expression. | |||
| CD86 | Monocytes, activated B cells, dendritic cells | 80 | Ligand for CD28 and CTLA4 |
| CD206 | Macrophages, endothelial cells | 175-190 | Type I membrane glycoprotein; only known example of a C-type lectin that contains multiple C-type CRDs (carbohydrate-recognition domains); it binds high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi |
