Multi - color Flow Cytometry Mini Panel for T/NK/NKT Cell Identification
10 Jun 2025
by AntBio
T cells (T Lymphocytes) are central effector cells of the adaptive immune system.
Natural Killer (NK) cells are lymphocytes that play a significant role in the innate immune system. They can directly kill virus - infected cells, tumor cells, and other abnormal cells without prior antigen stimulation, serving a critical role in immune surveillance and defense.
Natural Killer T (NKT) cells are a unique subset of T lymphocytes that share characteristics of both T cells and NK cells.
Human Panel Recommendations
| Marker | Fluorochrome | Max EX/EM (nm) | Corresponding Laser | Filter | Product Number | Format (1 T represents one test) |
|---|---|---|---|---|---|---|
| CD45 | APC - Cy7 | 659/782 | Red Laser | 780/60 | S0B1583 | 20/50/100/500 T available |
| CD3 | Alexa Fluor® 647 | 653/669 | Red Laser | 660/20 | S0B0155 | 20/50/100/500 T available |
| CD56 | PE | 496, 566/576 | Blue or Yellow - Green Laser | 575/26 | abs182503 | 25/100 T available |
Mouse Panel Recommendations
| Marker | Fluorochrome | Max EX/EM (nm) | Corresponding Laser | Filter | Product Number | Format (1 T represents one test) |
|---|---|---|---|---|---|---|
| CD45 | APC - Cy7 | 659/782 | Red Laser | 780/60 | S0B1692 | 20/50/100/500 T available |
| CD3 | FITC | 494/518 | Blue Laser | 530/30 | S0B1783 | 20/100/500 T available |
| CD49b (strains A, AKR, BALB/C, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, NOD, SJL, 129) | APC | 651/660 | Red Laser | 660/20 | 560628 | 50 μg |
| NK1.1 (strains C57BL, FVB/N, NZB) | APC | 651/660 | Red Laser | 660/20 | S0B5197 | 20/50/100/500 T available |
Notes:
CD45检测在人和小鼠样本中的主要目的是区分白细胞与非白细胞。在检测非免疫组织样本时必须添加，在需要确定目标细胞占总免疫细胞比例时也必须包含。
Detailed Explanation of Each Antibody Marker's Function:
|
CD antigen |
Cellular expression |
Molecular weight (kDa) |
Functions |
|
CD45 |
All hematopoietic cells |
180–240 (multiple isoforms) |
Tyrosine phosphatase, augments signaling through antigen receptor of B and T cells, multiple isoforms result from alternative splicing (see below) |
|
CD3 |
Thymocytes, T cells |
γ:25–28, δ:20, ε:20 |
Associated with the T-cell antigen receptor (TCR). Required for cellsurface expression of and signal transduction by the TCR |
|
CD56 |
NK cells |
135–220 |
Isoform of neural cell-adhesion molecule (NCAM), adhesion molecule |
|
CD49b |
B cells, monocytes, platelets, megakaryocytes, neuronal, epithelial and endothelial cells, osteoclasts |
160 |
α2 integrin, associates with CD29, binds collagen, laminin |
|
NK1.1 |
NK cells, T cells |
44 |
Regulates NK cytotoxicity |
