Multi - color Flow Cytometry Mini Panel for Macrophage Identification

10 Jun 2025 by AntBio

MDSCs are derived from bone marrow progenitor cells and immature myeloid cells (IMCs). Normally, they are precursors to dendritic cells, macrophages, and granulocytes. They can rapidly differentiate into mature granulocytes, DCs, and macrophages, which then migrate to respective organs and tissues to perform normal immune functions. IMCs constitute approximately 0.5% of peripheral blood mononuclear cells. However, under pathological conditions like tumors, infections, inflammation, sepsis, and surgical trauma, the maturation of these myeloid - derived precursor cells is blocked due to cytokines. As a result, they remain at various differentiation stages and become MDSCs with immune - suppressive functions. Acting on cytokines, MDSCs are recruited, migrate, and expand. Their quantity and proportion in peripheral blood increase by about tenfold, accounting for roughly 10% of peripheral blood mononuclear cells in patients and spanning the entire disease - development process.

Panel Recommendations

Marker	Fluorochrome	Max EX/EM (nm)	Corresponding Laser	Filter	Product Number	Format (1 T represents one test)
CD11b	PerCP - Cy5.5	482/676	Blue Laser	695/40	S0B5160	20/50/100/500 T available
GR - 1	APC - Cy7	659/782	Red Laser	780/60	S0B1702	20/50/100/500 T available
LY - 6C	FITC	494/518	Blue Laser	530/30	S0B5110	20/50/100/500 T available
LY - 6G	APC	651/660	Red Laser	660/20	S0B5168	20/50/100/500 T available
CD115 (optional)	PE	496, 566/576	Blue or Yellow - Green Laser	575/26	565249	100 μg

Notes:

As PMN - MDSCs are hard to distinguish from neutrophils, consider adding CD115. Murine PMN - MDSCs express higher levels of CD115 than neutrophils.

Detailed Explanation of Each Antibody Marker's Function:

CD antigen	Cellular expression	Molecular weight (kDa)	Functions
CD11b	Myeloid and NK cells	170	αM subunit of integrin CR3 (associated with CD18): binds CD54, complement component iC3b, and extracellular matrix proteins
LY-6G	Neutrophils (highly expressed), some inflammatory monocytes	25	Promote migration to the site of inflammation，regulate respiratory burst (ROS production), mediating NETosis (neutrophil extracellular trap)
GR-1	myeloid cells	14-30（complex）	Ly-6G/Ly-6C complex
LY-6C	Widely expressed in various myeloid cells, including monocytes, granulocyte precursors, and immature myeloid cells. Partial effects/memory T cells	14 - 18	Ly-6Chi monocytes migrate to the site of inflammation and differentiate into M1 macrophages. Ly-6C+T cells have stronger effector functions, such as IFN - γ secretion.
CD115	Monocytes, macrophages	150	Macrophage colony-stimulating factor (M-CSF) receptor