IHC shows positive staining in paraffin-embedded human stomach. Anti-ZO-1 Receptor antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ZO-1 Recombinant Rabbit mAb (S-2188-12)
ZO-1 Recombinant Rabbit mAb (S-2188-12)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ZO-1 Receptor |
| Synonyms | Tight junction protein 1; Tight junction protein ZO-1; Zona occludens protein 1; Zonula occludens protein 1; ZO1; TJP1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | Q07157 |
| Clone Number | S-2188-12 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:250-1:1000 | Hu, Ms |
Background
The ZO-1 receptor protein is a crucial component of tight junctions, which are essential for maintaining cell polarity and regulating the passage of substances across epithelial and endothelial cell layers. ZO-1, also known as zonula occludens-1, is a peripheral membrane protein that links transmembrane proteins of tight junctions to the actin cytoskeleton. It plays a significant role in the assembly and maintenance of tight junctions, thereby contributing to the barrier function of tissues. Additionally, ZO-1 is involved in various cellular processes such as cell signaling and proliferation, and its dysregulation has been implicated in several pathological conditions including cancer and inflammatory diseases.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-ZO-1 Receptor antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ZO-1 Receptor antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ZO-1 Receptor antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-ZO-1 Receptor antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
