WB result of Wnt3a Recombinant Rabbit mAb
Primary antibody: Wnt3a Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: L Wnt-3A whole cell lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 38 kDa
Wnt3a Recombinant Rabbit mAb (S-3300)
Wnt3a Recombinant Rabbit mAb (S-3300)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Wnt3a |
| Synonyms | Protein Wnt-3a; WNT3A |
| Location | Secreted |
| Accession | P56704 |
| Clone Number | S-3300 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:20000 | Ms |
| IHC-P | 1:200 | Ms |
| ICC | 1:200 | Ms |
Background
Wnt3a is a secreted, palmitoleated glycoprotein of ~39 kDa that acts as a principal ligand for canonical Wnt signaling; upon binding to Frizzled and LRP5/6 co-receptors it triggers a β-catenin–dependent cascade leading to TCF/LEF-mediated transcription of genes that govern embryonic axis formation, neural crest specification, self-renewal of hematopoietic and intestinal stem cells, and osteoblast differentiation, while its dysregulation drives colorectal, breast and hematologic malignancies, and recombinant Wnt3a is widely used in vitro to expand organoids and maintain pluripotency.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded E14.5 mouse embryo. Anti-Wnt3a antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in L Wnt-3A cells. Anti-Wnt3a antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
