Vitamin D Receptor Recombinant Rabbit mAb (S-1719-7)

Catalog Number: S0B1146 Application: WB, ICC, IP Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Vitamin D Receptor
Synonyms	Vitamin D3 receptor; VDR; 1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1; NR1I1
Immunogen	Synthetic Peptide
Location	Nucleus, Cytoplasm
Accession	P11473
Clone Number	S-1719-7
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, ICC, IP
Reactivity	Hu, Ms, Rt
Positive Sample	HeLa, T-47D, HL-60, DU 145, THP-1, HT-29, LOVO, NIH/3T3, mouse kidney, rat kidney
Predicted Reactivity	Bv, Pg, CtTa, Ck
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
ICC	1:500	Hu

Background

VDR is a nuclear transcription factor encoded by the VDR gene, which is a member of the steroid-thyroid-vitamin D receptor gene superfamily of nuclear transcription factors. It has multiple domains, including an amino-terminal segment (domains A and B), a DNA binding domain (C) with two zinc fingers, a hinge region (D), and a carboxyl terminal domain (E) with ligand and retinoid X receptor α (RXRα) binding sites. VDR acts as a ligand-activated transcription factor that binds to the vitamin D response element (VDRE) in the promoter regions of target genes, regulating their transcription. VDR is widely expressed in various human tissues, including the intestine, kidney, bone, skin, and immune cells. It has been reported to have more than 1000 target genes and is found in most human tissues. VDR is involved in calcium and phosphorus homeostasis, bone health, immune regulation, and has been implicated in the pathology of at least 17 types of cancer, autoimmune diseases, diabetes, osteoarthritis, and periodontal disease. It also plays a role in the central nervous system, with VDR gene polymorphisms and vitamin D deficiency being associated with an increased susceptibility to Alzheimer's disease and Parkinson's disease.

Picture

Western Blot

WB result of Vitamin D Receptor Recombinant Rabbit mAb
Primary antibody: Vitamin D Receptor Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: T-47D whole cell lysate 20 µg
Lane 3: HL-60 whole cell lysate 20 µg
Lane 4: DU 145 whole cell lysate 20 µg
Lane 5: THP-1 whole cell lysate 20 µg
Lane 6: HT-29 whole cell lysate 20 µg
Lane 7: LOVO whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDa

WB result of Vitamin D Receptor Recombinant Rabbit mAb
Primary antibody: Vitamin D Receptor Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDa

WB result of Vitamin D Receptor Recombinant Rabbit mAb
Primary antibody: Vitamin D Receptor Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDa

IP

Vitamin D Receptor Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Vitamin D Receptor in 0.4 mg T-47D whole cell lysate.
Western blot was performed on the immunoprecipitate using Vitamin D Receptor Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: T-47D whole cell lysate 40 µg (Input)
Lane 2: Vitamin D Receptor Recombinant Rabbit mAb IP in T-47D whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in T-47D whole cell lysate
Predicted MW: 48 kDa
Observed MW: 48 kDa

Immunocytochemistry

ICC shows positive staining in A431 cells. Anti-Vitamin D Receptor antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).