IHC shows positive staining in paraffin-embedded human kidney. Anti- VEGF Receptor 2/FLK-1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
VEGF Receptor 2/FLK-1 Recombinant Rabbit mAb (S-R400)
VEGF Receptor 2/FLK-1 Recombinant Rabbit mAb (S-R400)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1 (FLK-1), Kinase insert domain receptor (KDR), Protein-tyrosine kinase receptor flk-1, CD309, KDR |
| Location | Cell membrane |
| Accession | P35968 |
| Clone Number | S-R400 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:100 | null |
Background
VEGF Receptor 2 protein, also known as VEGFR-2 or Flk-1, is a receptor tyrosine kinase that plays a crucial role in angiogenesis, vascular development, and embryonic hematopoiesis. It is the main receptor for vascular endothelial growth factors (VEGFs), such as VEGFA, VEGFC, and VEGFD. VEGFR-2 is expressed widely, particularly in vascular endothelial cells and hemangioendotheliomas. It is localized in the endoplasmic reticulum and cell membrane. VEGFR-2 mediates the VEGF-induced proliferation, survival, migration, tube formation, and sprouting of endothelial cells, which are critical steps in angiogenesis and vascular development. The main function of VEGFR-2 is to regulate the growth and development of endothelial cells through signal transduction. This process involves dynamic interactions between various proteins and signaling pathways. Some of the major signaling pathways associated with VEGFR-2 include the PI3K-Akt, Ras-Raf-MEK-ERK, PKC-STAT3, and JAK2-STAT3 pathways.
Picture
Picture
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human placenta. Anti- VEGF Receptor 2/FLK-1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti- VEGF Receptor 2/FLK-1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- VEGF Receptor 2/FLK-1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
