WB result of UGGT1 Recombinant Rabbit mAb
Primary antibody: UGGT1 Recombinant mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: MCF-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 177 kDa
Observed MW: 170 kDa
UGGT1 Recombinant Rabbit mAb (S-1079-19)
UGGT1 Recombinant Rabbit mAb (S-1079-19)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | UGGT1 |
| Synonyms | UDP-glucose:glycoprotein glucosyltransferase 1; UGT1; hUGT1; UDP--Glc:glycoprotein glucosyltransferase; UDP-glucose ceramide glucosyltransferase-like 1 |
| Immunogen | Synthetic Peptide |
| Location | Endoplasmic reticulum |
| Accession | Q9NYU2 |
| Clone Number | S-1079-19 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 | |
| IHC-P | 1:500 |
Background
UGGT1, known as UDP-Glucose Glycoprotein Glucosyltransferase 1, is an enzyme involved in the process of protein glycosylation. It is a soluble protein in the endoplasmic reticulum (ER) that selectively reglycosylates unfolded glycoproteins, providing quality control for protein trafficking out of the ER. In the process of hepatitis C virus (HCV) entry into cells, UGGT1 has been identified as a host factor that affects HCV entry by influencing SR-BI. As a biomarker, UGGT1 has potential applications in the diagnosis and treatment of cervical diseases such as cervical intraepithelial neoplasia and cervical squamous cell carcinoma.
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Western Blot
IP
UGGT1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating UGGT1 in 0.4 mg Jurkat whole cell lysate.
Western blot was performed on the immunoprecipitate using UGGT1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate 5 µg (Input)
Lane 2: UGGT1 Rabbit mAb IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
Predicted MW: 177 kDa
Observed MW: 170 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- UGGT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti- UGGT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti- UGGT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- UGGT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- UGGT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
