WB result of UBR2 Recombinant Rabbit mAb
Primary antibody: UBR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: BxPC-3 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 200 kDa
Observed MW: 200 kDa
UBR2 Recombinant Rabbit mAb (S-2041-163)
UBR2 Recombinant Rabbit mAb (S-2041-163)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | UBR2 |
| Synonyms | E3 ubiquitin-protein ligase UBR2; N-recognin-2; Ubiquitin-protein ligase E3-alpha-2; Ubiquitin-protein ligase E3-alpha-II; C6orf133; KIAA0349; UBR2 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q8IWV8 |
| Clone Number | S-2041-163 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | 293T, Jurkat, BxPC-3, HeLa, NIH/3T3, mouse liver, C6, COS-7 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| ICC | 1:100 | Hu |
Background
UBR2 is an evolutionarily conserved E3 ubiquitin ligase of the N-end rule pathway that selectively recognizes and ubiquitinates proteins bearing specific N-terminal destabilizing residues, thereby targeting them for proteasomal degradation; it contains an N-terminal UBR box that binds type-1 (basic) and type-2 (bulky hydrophobic) N-degrons, a central RING finger domain that coordinates ubiquitin-charged E2 enzymes, multiple auto-inhibitory and substrate-recognition motifs, and C-terminal regions that mediate interactions with transcription factors, chromatin remodelers, and DNA damage response proteins, enabling UBR2 to regulate diverse processes such as spermatogenesis, neurogenesis, cardiac development, genomic stability, and metabolic homeostasis through both proteolytic and non-proteolytic ubiquitylation events.
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Western Blot
WB result of UBR2 Recombinant Rabbit mAb
Primary antibody: UBR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 200 kDa
Observed MW: 200 kDa
WB result of UBR2 Recombinant Rabbit mAb
Primary antibody: UBR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 200 kDa
Observed MW: 200 kDa
WB result of UBR2 Recombinant Rabbit mAb
Primary antibody: UBR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 200 kDa
Observed MW: 200 kDa
Immunocytochemistry
ICC shows positive staining in 293T cells. Anti-UBR2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
