WB result of TRIM63 Recombinant Rabbit mAb
Primary antibody: TRIM63 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Lane 3: mouse skeletal muscle lysate 20 µg
Negative control: mouse liver lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 40 kDa
TRIM63 Recombinant Rabbit mAb (S-2304-110)
TRIM63 Recombinant Rabbit mAb (S-2304-110)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TRIM63 |
| Synonyms | E3 ubiquitin-protein ligase TRIM63; Iris RING finger protein; Muscle-specific RING finger protein 1 (MuRF-1; MuRF1); RING finger protein 28; RING-type E3 ubiquitin transferase TRIM63; Striated muscle RING zinc finger protein; Tripartite motif-containing protein 63; IRF; MURF1; RNF28; SMRZ |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q969Q1 |
| Clone Number | S-2304-110 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | mouse heart, mouse skeletal muscle, rat heart, rat skeletal muscle |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Ms, Rt |
| IHC-P | 1:200-1:5000 | Hu, Ms, Rt |
Background
TRIM63, also known as MuRF1 (Muscle RING Finger 1), is a muscle-specific E3 ubiquitin ligase encoded by the TRIM63 gene, primarily expressed in cardiac and skeletal muscle, where it plays a critical role in regulating muscle protein homeostasis by ubiquitinating sarcomeric proteins such as MYH6, MYBPC3, and cardiac troponin I for proteasomal degradation, thereby influencing muscle atrophy and hypertrophy; structurally, TRIM63 belongs to the tripartite motif (TRIM) family and contains an N-terminal RING domain, B-box, coiled-coil motifs, and a C-terminal acidic tail, and mutations in TRIM63 have been implicated in human hypertrophic cardiomyopathy due to impaired E3 ligase activity and disrupted protein degradation pathways.
Picture
Picture
Western Blot
WB result of TRIM63 Recombinant Rabbit mAb
Primary antibody: TRIM63 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Lane 2: rat skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 40 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-TRIM63 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-TRIM63 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-TRIM63 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-TRIM63 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-TRIM63 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
