IHC shows positive staining in paraffin-embedded human skin. Anti-TLR4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
TLR4 Recombinant Mouse mAb (SDT-3305)
TLR4 Recombinant Mouse mAb (SDT-3305)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | TLR4 |
| Synonyms | Toll-like receptor 4; CD284; Lps; Tlr4 |
| Location | Endosome, Cell membrane |
| Accession | Q9QUK6 |
| Clone Number | SDT-3305 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | IHC-P |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Pig |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:250 | Hu |
Background
Toll-like receptor 4 (TLR4) is a type I transmembrane pattern-recognition receptor expressed predominantly on macrophages, dendritic cells, and epithelial barriers; upon recognition of lipopolysaccharide from Gram-negative bacteria in complex with MD-2 and CD14, it homodimerizes and initiates a MyD88- and TRIF-dependent signaling cascade that activates NF-κB, MAPKs, and IRF3, driving the transcription of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), type I interferons, and co-stimulatory molecules essential for innate immunity and the subsequent shaping of adaptive responses, while excessive or dysregulated TLR4 activation contributes to septic shock, chronic inflammatory disorders such as atherosclerosis and metabolic syndrome, and ischemia–reperfusion injury, making it a therapeutic target for antagonists like lipid A analogs and monoclonal antibodies.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human salivary gland. Anti-TLR4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-TLR4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
