WB result of TdT Recombinant Rabbit mAb
Primary antibody: TdT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: Molt-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 56 kDa
TdT Recombinant Rabbit mAb (S-2364-59)
TdT Recombinant Rabbit mAb (S-2364-59)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TdT |
| Synonyms | DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase (Terminal transferase); TDT; DNTT |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | P04053 |
| Clone Number | S-2364-59 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Positive Sample | Jurkat, Molt-4 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
Terminal deoxynucleotidyl transferase (TdT), also called DNA nucleotidylexotransferase or terminal transferase, is a template-independent DNA polymerase encoded by the DNTT gene on human chromosome 10q23-q24 that is selectively expressed in immature lymphoid precursors (pre-B and pre-T cells) and certain leukemias/lymphomas; it catalyzes the non-templated addition of deoxynucleotides to the 3'-OH ends of DNA, generating N-region diversity at V(D)J junctions during immunoglobulin and T-cell receptor gene rearrangement, thereby expanding antigen-receptor repertoires critical for adaptive immunity, and can utilize a broad range of divalent cations (Mg²⁺, Mn²⁺, Zn²⁺, Co²⁺) as cofactors .
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in MOLT-4 cells. Anti- TdT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
