WB result of TCF1/TCF7 Recombinant Rabbit mAb
Primary antibody: TCF1/TCF7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: Molt-4 whole cell lysate 20 µg
Lane 3: COLO 205 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 38-46 kDa
TCF1/TCF7 Recombinant Rabbit mAb (S-3486)
TCF1/TCF7 Recombinant Rabbit mAb (S-3486)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TCF1/TCF7 |
| Synonyms | Transcription factor 7; TCF-7; T-cell-specific transcription factor 1 (T-cell factor 1; TCF-1) |
| Location | Nucleus |
| Accession | P36402 |
| Clone Number | S-3486 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms |
| Positive Sample | Jurkat, Molt-4, COLO 205, EL4, mouse thymus |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms |
| IHC-P | 1:200-1:500 | Hu, Ms, Rt |
Background
TCF1 (T-cell factor 1), encoded by the TCF7 gene, is a 48–58 kDa sequence-specific HMG-box transcription factor and a founding member of the TCF/LEF family that serves as the primary nuclear effector of the canonical Wnt/β-catenin pathway, binding via its N-terminal β-catenin interaction domain and its central DNA-binding HMG motif to the consensus sequence 5’-(A/T)(A/T)CAAAG-3’ in promoters/enhancers of genes such as MYC, CCND1 and LEF1, thereby driving embryonic stem cell pluripotency, thymic T-cell lineage specification, CD8⁺ T-cell memory formation, and adult intestinal epithelial renewal, while its expression is dynamically regulated by alternative promoters and differential splicing to yield long (p45) and short (p33) isoforms with opposing activities; TCF1 also possesses context-dependent repressive functions when β-catenin is absent, interacts with Groucho/TLE co-repressors, and is frequently inactivated by loss-of-function mutations or deletions in colorectal, hematopoietic and other malignancies, making it both a developmental gatekeeper and a tumor suppressor.
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Western Blot
WB result of TCF1/TCF7 Recombinant Rabbit mAb
Primary antibody: TCF1/TCF7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Lane 2: EL4 whole cell lysate 20 µg
Lane 3: mouse thymus lysate 20 µg
Negative control: A20 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 40-50 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-TCF1/TCF7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TCF1/TCF7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-TCF1/TCF7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-TCF1/TCF7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-TCF1/TCF7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded NK/T-cell lymphoma. Anti-TCF1/TCF7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-TCF1/TCF7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-TCF1/TCF7 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
