WB result of SEK1/MKK4 Rabbit pAb
Primary antibody: SEK1/MKK4 Rabbit pAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 40 kDa
SEK1/MKK4 Rabbit Polyclonal Antibody
SEK1/MKK4 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SEK1/MKK4 |
| Synonyms | Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4; JNK-activating kinase 1; MAPK/ERK kinase 4 (MEK 4); SAPK/ERK kinase 1 (SEK1); Stress-activated protein kinase kinase 1 (SAPK kinase 1; SAPKK-1; SAPKK1); c-Jun N-terminal kinase kinase 1 (JNKK); JNKK1; MEK4; PRKMK4; SERK1; SKK1; MAP2K4 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P45985 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HEK-293, HeLa, K562, Jurkat, NIH/3T3, mouse brain, C6, rat brain, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:200 | Hu |
Background
SEK1/MKK4 (also known as MAP2K4, MEK4, JNKK1) is a 399-amino-acid dual-specificity protein kinase located on chromosome 17p11.2 that functions as the immediate upstream activator of both JNK and p38 MAPKs in response to diverse extracellular stresses such as osmotic shock, UV radiation, and cytokines; after phosphorylation by upstream MAP3Ks like MEKK1/2, SEK1/MKK4 phosphorylates JNK and p38 on their Thr-Pro-Tyr motifs, thereby linking stress signals to transcriptional and apoptotic programs, and its activity and stability are subjected to negative feedback control through JNK-mediated phosphorylation of the E3 ubiquitin ligase Itch, which then ubiquitinates and targets SEK1/MKK4 for proteasomal degradation .
Picture
Picture
Western Blot
WB result of SEK1/MKK4 Rabbit pAb
Primary antibody: SEK1/MKK4 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 42 kDa
WB result of SEK1/MKK4 Rabbit pAb
Primary antibody: SEK1/MKK4 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 42 kDa
WB result of SEK1/MKK4 Rabbit pAb
Primary antibody: SEK1/MKK4 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 42 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-SEK1/MKK4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SEK1/MKK4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-SEK1/MKK4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
