WB result of DAZL Recombinant Rabbit mAb
Primary antibody: DAZL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Negative control: mouse brain lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 38 kDa
S-RMab® DAZL Recombinant Rabbit mAb (S-1588-3)
S-RMab® DAZL Recombinant Rabbit mAb (S-1588-3)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | DAZL |
| Synonyms | Deleted in azoospermia-like; DAZ-like autosomal; Deleted in azoospermia-like 1; Dazl1; Dazla; Dazl |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q64368 |
| Clone Number | S-1588-3 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Ms, Rt |
| Positive Sample | Mouse testis, rat testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:200 | Ms, Rt |
| IF | 1:2000 | Ms |
Background
DAZL (Deleted in Azoospermia-Like) is a highly conserved, germ-cell-specific RNA-binding protein that orchestrates key post-transcriptional events across vertebrate gametogenesis. It carries an N-terminal RNA-recognition motif and a C-terminal DAZ repeat that jointly bind U-rich sequences in the 3′ UTRs of target mRNAs, enabling DAZL to recruit poly(A)-binding proteins (PABPs) and stimulate cap-dependent translation initiation independently of the poly(A) tail length. Expressed from primordial germ cell (PGC) specification through meiotic progression in both sexes, DAZL licenses these cells to respond to retinoic acid, thereby promoting meiotic entry, synaptonemal complex formation via SYCP3, and spindle assembly during oocyte maturation. In vivo, loss of DAZL causes complete germ-cell depletion and infertility, whereas haploinsufficiency yields abnormal sperm and ovarian insufficiency, underscoring its role as a master regulator of germline survival and differentiation.
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Picture
Western Blot
WB result of DAZL Recombinant Rabbit mAb
Primary antibody: DAZL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat testis lysate 20 µg
Negative control: rat brain lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 38 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse testis. Anti-DAZL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-DAZL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-DAZL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat cerebral cortex. Anti-DAZL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse testis. Anti-DAZL antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
