2μg (R: reducing condition, N: non-reducing condition).
Recombinant Carboxypeptidase B，Bovine
Recombinant Carboxypeptidase B，Bovine
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$146 USD
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Product Details
Product Details
Product Specification
| Species | Bovine |
| Synonyms | Carboxypeptidase B |
| Expression System | PichiaPastoris |
| Molecular Weight | 33 kD |
| Purity | ＞95% by SDS-PAGE & HPLC |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris-HCl 、100mM NaCl |
| Reconstitution | Carboxypeptidase B lyophilized powder was dissolved in sterile water or 25mM Tris-HCl (pH7.6) to make the concentration of enzyme solution 1-10mg /ml. |
| Stability & Storage | ·24 month, 2 to 8 °C under sterile conditions |
| Reference | [1] Akparov V, Sokolenko N, Timofeev V, Kuranova I. Structure of the complex of carboxypeptidase B and N-sulfamoyl-L-arginine. Acta Crystallogr F Struct Biol Commun. 2015 Oct;71(Pt 10):1335-40. |
Background
Carboxypeptidase B, also known as peptidyl-L-lysine (L-arginine) hydrolase, contains one Zn atom per molecule and can selectively hydrolyze arginine and lysine at the carboxyl end of proteins or polypeptides. CPB can be used in the production of recombinant insulin and its analogues, and the quality detection of recombinant antibodies. and also used in the production of other recombinant polypeptides.
Components
20mM Tris-HCl 、100mM NaCl、Mannitol、Carboxypeptidase B
Protocol
The recommended amount of Carboxypeptidase B is 1:50~1:1000 (w/w) compared with the target protein (mass ratio), the optimal pH is 7.5-9.0, and the appropriate enzyme digestion temperature is 25℃~37℃.
Guidelines
1. For different protein samples, it is necessary to explore the optimal enzyme concentration and reaction time.
2. Reaction at 25℃~37℃, the reaction time can be appropriately extended when digestion at 25℃.
Unit Definition
The amount of enzyme that catalyzes the hydrolysis of 1 μmol of maroyl-L-arginine in 1 min is one enzyme activity unit of carboxypeptidase B.
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SDS-PAGE
SEC-HPLC
RP-HPLC
