WB result of Rac1/2/3 Recombinant Rabbit mAb
Primary antibody: Rac1/2/3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HUVEC whole cell lysate 20 µg
Lane 4: SW480 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 22, 24 kDa
Rac1/2/3 Recombinant Rabbit mAb (S-2720-10)
Rac1/2/3 Recombinant Rabbit mAb (S-2720-10)
Price:
Regular price
$131.00 SGD
Regular price
Sale price
$131.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Rac1/2/3 |
| Synonyms | GX; Small G protein; p21-Rac2; RAC2; Ras-like protein TC25; p21-Rac1; RAC1; TC25; p21-Rac3; RAC3 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P15153、P63000、P60763 |
| Clone Number | S-2720-10 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HEK-293, HeLa, HUVEC, SW480, RAW264.7, NIH/3T3, C2C12, mouse brain, PC-12, C6, rat brain |
| Predicted Reactivity | Bv, GP |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
| ICC | 1:100 | Hu, Ms, Rt |
Background
Rac1/2/3 are highly homologous Rho-family small GTPases that cycle between an inactive GDP-bound and an active GTP-bound state to act as molecular switches relaying extracellular cues to the actin cytoskeleton, thereby orchestrating cell migration, adhesion, membrane ruffling, phagocytosis, and NADPH oxidase–mediated ROS production; while Rac1 is ubiquitously expressed and essential for lamellipodium formation, Rac2 is hematopoietic-specific and predominantly regulates neutrophil ROS generation and chemotaxis, and Rac3, enriched in brain and muscle, fine-tunes neurite outgrowth and synaptic plasticity, yet all three isoforms share overlapping upstream regulators (e.g., Vav, Tiam1, P-Rex1) and downstream effectors (e.g., PAKs, WAVE, formins) and are frequently hyperactivated in cancer, immune disorders, and cardiovascular disease, making them attractive therapeutic targets.
Picture
Picture
Western Blot
WB result of Rac1/2/3 Recombinant Rabbit mAb
Primary antibody: Rac1/2/3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: C2C12 whole cell lysate 20 µg
Lane 4: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 22, 24 kDa
WB result of Rac1/2/3 Recombinant Rabbit mAb
Primary antibody: Rac1/2/3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 22, 24 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Rac1/2/3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Rac1/2/3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Rac1/2/3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HEK293 cells. Anti- Rac1/2/3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in RAW 264.7 cells. Anti- Rac1/2/3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in C6 cells. Anti- Rac1/2/3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
