Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The EC50 for this effect is less than 20ng/mL in the presence of 5ng/mL Recombinant Human Wnt‑3a.
R-spondin3/RSPO3 Protein, Mouse
R-spondin3/RSPO3 Protein, Mouse
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$496 USD
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | R-spondin-3; RSPO3; Protein with TSP type-1 repeat; Roof plate-specific spondin-3; Thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2; CRISTIN1 |
| Accession | Q2TJ95 |
| Amino Acid Sequence | Met33-His277 |
| Expression System | HEK293 |
| Molecular Weight | 45 kDa (Reducing) |
| Purity | >95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Kim KA, Zhao J, Andarmani S, et al. R-Spondin proteins: a novel link to beta-catenin activation. Cell Cycle. 2006;5(1):23-26. |
Background
R-spondin3 is a member of the R-spondin family of secreted proteins that play crucial roles in regulating Wnt/β-catenin signaling pathway. R-spondin3 is known to enhance Wnt signaling by binding to the LGR4-6 receptors, leading to the stabilization of the Wnt receptor complex and promoting the nuclear translocation of β-catenin. This results in the activation of Wnt target genes involved in various cellular processes such as proliferation, differentiation, and stem cell maintenance. R-spondin3 has been implicated in embryonic development, tissue homeostasis, and regeneration, as well as in the pathogenesis of certain diseases, including cancer.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
ELISA
Measured by its binding ability in a functional ELISA. When Recombinant Mouse RNF43 mFC Chimera, is immobilized 2µg/mL (100 µl/well), Recombinant Mouse R-spondin3/RSPO3 His Tag binds with an EC50 of 0.02-0.036μg/ml.
