WB result of PSAP Recombinant Rabbit mAb
Primary antibody: PSAP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 75 kDa
PSAP Recombinant Rabbit mAb (S-2399-11)
PSAP Recombinant Rabbit mAb (S-2399-11)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PSAP |
| Synonyms | Prosaposin; Proactivator polypeptide; GLBA; SAP1 |
| Immunogen | Synthetic Peptide |
| Location | Lysosome |
| Accession | P07602 |
| Clone Number | S-2399-11 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | PANC-1, PC-3, HeLa |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
Background
Prosaposin (PSAP) is a highly conserved, 68- to 73-kDa glycoprotein encoded by the PSAP gene on chromosome 10q22.1 that serves dual intracellular and extracellular roles: inside the endolysosomal system it is proteolytically cleaved into four small lysosomal activator proteins (saposins A–D) that bind either specific hydrolases or their glycosphingolipid substrates to enable sphingolipid catabolism, while outside the cell PSAP acts as a secreted neurotrophic factor that supports neuronal survival, neurite outgrowth and ERK signaling, can be endocytosed via LRP1 or CI-M6PR, and has recently been implicated in cancer progression, metastasis and as an emerging therapeutic target in tumors.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-PSAP antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-PSAP antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-PSAP antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-PSAP antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
