WB result of Phospho-PLCγ1 (Tyr783) Recombinant Rabbit mAb
Primary antibody: Phospho-PLCγ1 (Tyr783) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Jurkat whole cell lysate 20 µg
Lane 2: Jurkat treated with 50 mM pervanadate for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 149 kDa
Observed MW: 150 kDa
Phospho-PLCγ1 (Tyr783) Recombinant Rabbit mAb (S-1184-12)
Phospho-PLCγ1 (Tyr783) Recombinant Rabbit mAb (S-1184-12)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-PLCγ1 (Tyr783) |
| Synonyms | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II (PLC-II), Phospholipase C-gamma-1 (PLC-gamma-1), PLCG1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P19174 |
| Clone Number | S-1184-12 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Predicted Reactivity | Ms, Bv, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | |
| ICC | 1:500 |
Background
PLCG1, or Phospholipase C Gamma 1, is a crucial enzyme protein involved in cellular signaling, differentiation, proliferation, and various other biological processes. it is crucial in receptor-mediated tyrosine kinase activator signaling within cells. Receptor tyrosine kinases interact with SRC domains to enzymatically activate PLCγ1 via phosphorylation at tyrosine 783. Upon activation PLCG1 leads to the translocation of Ras guanine nucleotide exchange factor RasGRP1 to the Golgi apparatus, activating Ras. PLCG1 is also a primary substrate of heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinases.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Phospho-PLCγ1 (Tyr783) Recombinant Rabbit mAb
Lane 1: Phospho-PLCγ1 (Tyr783) peptide
Lane 2: PLCγ1 unmodified peptide
Primary antibody: Phospho-PLCγ1 (Tyr783) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunocytochemistry
ICC analysis of Jurkat cells treated with pervanadate (50mM, 5mins) (top panel) and Jurkat cells untreated with pervanadate (50mM, 5mins) (below panel). Anti- Phospho-PLCγ1 (Tyr783) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
