WB result of Phospho-IκBα (Ser32) Recombinant Rabbit mAb
Primary antibody: Phospho-IκBα (Ser32) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes and 20 ng/ml TNF-α for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 38 kDa
Phospho-IκBα (Ser32) Recombinant Rabbit mAb (S-1084-395)
Phospho-IκBα (Ser32) Recombinant Rabbit mAb (S-1084-395)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-IκBα (Ser32) |
| Synonyms | NF-kappa-B inhibitor alpha; I-kappa-B-alpha (IkB-alpha; IkappaBalpha); Major histocompatibility complex enhancer-binding protein MAD3; IKBA; MAD3; NFKBI; NFKBIA |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P25963 |
| Clone Number | S-1084-395 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms |
| Predicted Reactivity | Pg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms |
Background
Phospho-IκBα (Ser32) is a phosphorylated form of the IκBα protein, which plays a crucial role in the NF-κB signaling pathway. IκBα normally inhibits NF-κB by binding to it and preventing its nuclear translocation. However, when IκBα is phosphorylated at Ser32 (and often also at Ser36), it undergoes ubiquitination and subsequent degradation by the 26S proteasome, thereby releasing NF-κB to translocate to the nucleus and activate gene expression. This phosphorylation event is typically mediated by the IKK complex in response to various stimuli such as cytokines, growth factors, and stress signals. Antibodies specific to Phospho-IκBα (Ser32) are widely used in research to detect and quantify this phosphorylated protein, helping to elucidate the activation status of the NF-κB pathway in different cellular contexts.
Picture
Picture
Western Blot
WB result of Phospho-IκBα (Ser32) Recombinant Rabbit mAb
Primary antibody: Phospho-IκBα (Ser32) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 20 ng/ml TNF-α for 10 minutes and 100 nM Calyculin A for 10 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 38 kDa
This blot was developed with high sensitivity substrate
Dot Blot
Dot blot result of Phospho-IκBα (Ser32) Recombinant Rabbit mAb
Lane 1: IκBα (Ser32) phospho peptide
Lane 2: IκBα (Ser32) unmodified peptide
Primary antibody: Phospho-IκBα (Ser32) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
