WB result of Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb
Primary antibody: Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47, 51 kDa
Observed MW: 45, 51 kDa
Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb (S-1529-102)
Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb (S-1529-102)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-GSK3α/β (Ser21/9) |
| Synonyms | Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | P49840、 P49841 |
| Clone Number | S-1529-102 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms, Rt |
Background
Phospho-GSK3α/β (Ser21/9) refers to the phosphorylated forms of glycogen synthase kinase 3 alpha (GSK3α) at serine 21 and glycogen synthase kinase 3 beta (GSK3β) at serine 9. The phosphorylation at these sites is inhibitory, meaning it reduces the activity of these kinases. GSK3α/β are serine/threonine kinases that play crucial roles in various cellular processes, including cell fate determination, glucose homeostasis, and Wnt signaling. When phosphorylated at Ser21/9, the activity of GSK3α/β is suppressed, which can have significant impacts on these pathways. For example, in the Wnt signaling pathway, the inhibition of GSK3β by phosphorylation at Ser9 prevents the degradation of β-catenin, allowing it to accumulate and activate downstream gene expression. This phosphorylation is often regulated by upstream signals such as insulin or growth factors, which can induce the phosphorylation of GSK3α/β at these sites through various signaling cascades.
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Picture
Western Blot
WB result of Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb
Primary antibody: Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: Serum-starved NIH/3T3 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47, 51 kDa
Observed MW: 45, 55 kDa
WB result of Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb
Primary antibody: Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47, 51 kDa
Observed MW: 47, 51 kDa
Dot Blot
Dot blot result of Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb
Lane 1: GSK-3β (Ser9) phospho peptide
Lane 2: GSK-3β (Ser9) unmodified peptide
Primary antibody: Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
