WB result of Phospho-CrkL (Tyr207) Rabbit pAb
Primary antibody: Phospho-CrkL (Tyr207) Rabbit pAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: phosphatase treated K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 38 kDa
Phospho-CrkL (Tyr207) Rabbit Polyclonal Antibody
Phospho-CrkL (Tyr207) Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-CrkL (Tyr207) |
| Synonyms | Crk-like protein; CRKL |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P46109 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | K562 |
| Purification | Immunogen Affinity |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
Phospho-CrkL (Tyr207), the tyrosine-207-phosphorylated form of the CRK-like adaptor protein CrkL, is a 34 kDa signaling scaffold that contains SH2 and SH3 domains enabling it to bind multiple partners and propagate signals through MAPK, PI3K and STAT cascades; phosphorylation at Tyr207 (the CrkL counterpart of Tyr221 in CrkII) is a key regulatory switch controlling cytoskeletal reorganization, immune-cell signaling, cell proliferation and migration, and its dysregulation contributes to Philadelphia-chromosome-positive leukemias via constitutive activation with BCR-ABL, while developmental perturbations are linked to congenital heart defects, making p-CrkL(Tyr207) a focal point in oncogenic and developmental research .
Picture
Picture
Western Blot
