WB result of Perforin Recombinant Rabbit mAb
Primary antibody: Perforin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: CTLL-2 whole cell lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Negative control: C2C12 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 60-80 kDa
Perforin Recombinant Rabbit mAb (S-1944-68)
Perforin Recombinant Rabbit mAb (S-1944-68)
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$100.00 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Perforin |
| Synonyms | Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein; Pfp; Prf1 |
| Immunogen | Recombinant Protein |
| Location | Secreted, Cell membrane |
| Accession | P10820 |
| Clone Number | S-1944-68 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms, Rt |
| Positive Sample | CTLL-2, mouse spleen, rat spleen |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Ms, Rt |
Background
Perforin is a crucial protein involved in the immune response, primarily produced by cytotoxic T lymphocytes and natural killer cells. It functions by forming pores in the membranes of target cells, such as virus-infected or cancerous cells. Once released from immune cells, perforin molecules aggregate and insert into the target cell membrane, creating channels that disrupt the cell's osmotic balance, leading to cell lysis and death. This mechanism is essential for eliminating harmful cells and maintaining the body's overall health.
Picture
Picture
Western Blot
WB result of Perforin Recombinant Rabbit mAb
Primary antibody: Perforin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 62 kDa
