2e5 of transient transfected anti- MSLN ScFv CAR-293 cells were stained with 0.1ug PE-Labeled MSLN Fc&Avi Tag Protein, Human, (Cat. No. UA011284) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). PE signal was used to evaluate the binding activity.
2e5 of transient transfected anti- MSLN ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.
PE-Labeled MSLN Fc&Avi Tag Protein, Human
PE-Labeled MSLN Fc&Avi Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | CAK1 antigen,Pre-pro-megakaryocyte-potentiating factor |
| Accession | Q13421-1 |
| Amino Acid Sequence | Glu296-Gly580 with Human IgG1 Fc &Avi Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 72-85kDa (Reducing) |
| Conjugation | PE |
| Tag | Avi Tag, Human IgG1 Fc |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, pH7.4 |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.Hassan, R. et al: Mesothelin: a new target for immunotherapy, Clin.Cancer Res. 10:3937(2004). |
Background
Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF. MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation. The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
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