WB result of PDIA3 Rabbit pAb
Primary antibody: PDIA3 Rabbit pAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: Daudi whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HaCaT whole cell lysate 20 µg
Lane 5: 293T whole cell lysate 20 µg
Lane 6: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 62 kDa
PDIA3 Rabbit Polyclonal Antibody
PDIA3 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PDIA3 |
| Synonyms | Protein disulfide-isomerase A3; 58 kDa glucose-regulated protein; 58 kDa microsomal protein (p58); Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57 (ER protein 57; ERp57); Endoplasmic reticulum resident protein 60 (ER protein 60; ERp60); ERP57; ERP60; GRP58 |
| Immunogen | Synthetic Peptide |
| Location | Endoplasmic reticulum |
| Accession | P30101 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | HepG2, Daudi, HeLa, HaCat, 293T, PC-3, human colon, human colon cancer, human endometrium cancer, human lung cancer, human thyroid cancer |
| Predicted Reactivity | Bv, AfGrMk |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
| ICC | 1:500 | Hu |
Background
PDIA3 (Protein Disulfide Isomerase Family A Member 3), also known as ERp57 or GRP58, is a multifunctional enzyme belonging to the protein disulfide isomerase (PDI) family. It is predominantly located in the endoplasmic reticulum (ER) and acts as a molecular chaperone and redox catalyst. PDIA3 interacts with lectin chaperones like calreticulin and calnexin to facilitate the folding of newly synthesized glycoproteins by promoting the formation of disulfide bonds. Additionally, it prevents protein aggregation and plays a role in the unfolded protein response (UPR) during ER stress. Beyond its canonical functions, PDIA3 has been implicated in various biological processes, including viral infections, where it can influence viral entry and replication. In disease contexts, PDIA3 is often upregulated in cancers and has been associated with tumor progression and drug resistance. Recent studies also highlight its involvement in metabolic disorders, such as obesity, by regulating the function of adipose tissue macrophages.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-PDIA3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PDIA3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrium cancer. Anti-PDIA3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-PDIA3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-PDIA3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in Daudi cells. Anti- PDIA3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression Atlas
Expression of PDIA3 in tumor tissue.
Expression of PDIA3 in human tissue.
