WB result of PDGFR alpha Recombinant Rabbit mAb
Primary antibody: PDGFR alpha Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A172 whole cell lysate 20 µg
Lane 2: A204 whole cell lysate 20 µg
Lane 3: SH-SY5Y whole cell lysate 20 µg
Negative control: A172 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
PDGFR alpha Recombinant Rabbit mAb (SDT-3353)
PDGFR alpha Recombinant Rabbit mAb (SDT-3353)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PDGFR alpha |
| Synonyms | Platelet-derived growth factor receptor alpha; PDGF-R-alpha; PDGFR-alpha; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2 (PDGFR-2); CD140a; PDGFR2; RHEPDGFRA; PDGFRA |
| Location | Cell membrane |
| Accession | P16234 |
| Clone Number | SDT-3353 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A204, SH-SY5Y, NIH/3T3, mouse lung, rat brain, rat heart, rat lung |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
PDGFR alpha, CD140a (also called platelet-derived growth factor receptor-alpha, PDGFR-α) is a 170-kDa glycosylated cell-surface receptor tyrosine kinase encoded by the PDGFRA gene, featuring an extracellular ligand-binding region with five immunoglobulin-like domains, a single transmembrane segment and an intracellular split tyrosine-kinase domain; upon binding PDGF-AA, -AB or -BB it forms homo- or heterodimers, triggers downstream signalling cascades that drive proliferation, migration and differentiation of mesenchymal and glial cells, and is indispensable for embryogenesis of kidney, testis and other organs, wound healing and maintenance of hematologic tissues, while PDGFRA mutations or amplifications are linked to clonal hypereosinophilic neoplasms, gastrointestinal stromal tumours, gliomas and hepatocellular carcinoma, making CD140a a potential therapeutic target in these cancers .
Picture
Picture
Western Blot
WB result of PDGFR alpha Recombinant Rabbit mAb
Primary antibody: PDGFR alpha Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
WB result of PDGFR alpha Recombinant Rabbit mAb
Primary antibody: PDGFR alpha Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat heart lysate 20 µg
Lane 3: rat lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-PDGFR alpha antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PDGFR alpha antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse E14.5 intervertebral disc. Anti-PDGFR alpha antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse E14.5 lung. Anti-PDGFR alpha antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-PDGFR alpha antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-PDGFR alpha antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in A204 cells (top panel) and negative staining in A172 cells (below panel). Anti-PDGFR alpha antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
