WB result of PCK1 Recombinant Rabbit mAb
Primary antibody: PCK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COLO 205 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 69 kDa
PCK1 Recombinant Rabbit mAb (S-2350-30)
PCK1 Recombinant Rabbit mAb (S-2350-30)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PCK1 |
| Synonyms | Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEPCK-C; Serine-protein kinase PCK1; PEPCK1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Endoplasmic reticulum |
| Accession | P35558 |
| Clone Number | S-2350-30 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000- | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
PCK1, also known as phosphoenolpyruvate carboxykinase 1, is a crucial enzyme involved in gluconeogenesis and the conversion of oxaloacetate to phosphoenolpyruvate. It plays a significant role in maintaining glucose homeostasis by facilitating the production of glucose from non-carbohydrate sources. This enzyme is primarily expressed in tissues such as the liver and kidneys, where gluconeogenesis predominantly occurs. Abnormal regulation or activity of PCK1 has been implicated in metabolic disorders, including diabetes.
Picture
Picture
Western Blot
WB result of PCK1 Recombinant Rabbit mAb
Primary antibody: PCK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 69 kDa
WB result of PCK1 Recombinant Rabbit mAb
Primary antibody: PCK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 69 kDa
Immunocytochemistry
ICC shows positive staining in A549 cells. Anti-PCK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
