WB result of p27 Kip1 Recombinant Rabbit mAb
Primary antibody: p27 Kip1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Lane 6: Raji whole cell lysate 20 µg
Negative control: Daudi whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 24 kDa
p27 Kip1 Recombinant Rabbit mAb (SDT-361-310)
p27 Kip1 Recombinant Rabbit mAb (SDT-361-310)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | p27 Kip1 |
| Synonyms | Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Cdkn1b; KIP-1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus, Cytoplasm |
| Accession | P46414 |
| Clone Number | SDT-361-310 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Daudi, HeLa, MCF7, SH-SY5Y, Jurkat, Raji, NIH/3T3, C6 |
| Predicted Reactivity | Fg, Ct |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IP | 1:50 | Hu |
| IHC-P | 1:500 | Hu, Ms, Rt |
Background
p27 KIP1 is a member of the Cyclin Inhibitory Protein/Kinase Inhibitory Protein (CIP/KIP) family of cyclin-dependent kinase (Cdk) inhibitors. It plays roles in all phases of the cell division cycle. Its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes enforces the G1 restriction point. Thus, important cellular functions as the cell division cycle, respiration, RNA processing, translation, and cell adhesion, are under p27 KIP1 regulation. Loss or reduced expression is associated with poor outcome in carcinoma of breast, prostate, GI tract and lung.
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Picture
Western Blot
WB result of p27 Kip1 Recombinant Rabbit mAb
Primary antibody: p27 Kip1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22 kDa
WB result of p27 Kip1 Recombinant Rabbit mAb
Primary antibody: p27 Kip1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22 kDa
IP
p27 Kip1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating p27 Kip1 in 0.4 mg MCF7 whole cell lysate.
Western blot was performed on the immunoprecipitate using p27 Kip1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: MCF7 whole cell lysate 20 µg (Input)
Lane 2: p27 Kip1 Rabbit mAb IP in MCF7 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in MCF7 whole cell lysate
Predicted MW: 22 kDa
Observed MW: 27 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti- p27 Kip1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- p27 Kip1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti- p27 Kip1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti- p27 Kip1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti- p27 Kip1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti- p27 Kip1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti- p27 Kip1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti- p27 Kip1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
