WB result of NUT Recombinant Rabbit mAb
Primary antibody: NUT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 120 kDa
Observed MW: 150 kDa
NUT Recombinant Rabbit mAb (SDT-3062)
NUT Recombinant Rabbit mAb (SDT-3062)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NUT |
| Synonyms | NUT family member 1; Nuclear protein in testis; C15orf55; NUTM1 |
| Location | Cytoplasm, Nucleus |
| Accession | Q86Y26 |
| Clone Number | SDT-3062 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Rt |
| IHC-P | 1:250 | Hu, Rt |
| IF | 1:500 | Hu, Rt |
Background
The NUT protein, which stands for nuclear protein in testis, is a transcription factor that plays a crucial role in embryonic development. It is typically found in the testes and is involved in the regulation of gene expression. However, when it is abnormally expressed or translocated in other tissues, it can lead to the development of certain types of cancers, particularly NUT midline carcinoma (NMC), a rare but aggressive malignancy. The presence of the NUT protein in cancer cells is often associated with poor prognosis, and it has become an important target for research in cancer diagnostics and therapeutics.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-NUT antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human midline carcinoma. Anti-NUT antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-NUT antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded rat testis. Anti-NUT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
