IHC shows positive staining in paraffin-embedded human colon. Anti-MUC5B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
MUC5B Recombinant Rabbit mAb (SDT-2351-40)
MUC5B Recombinant Rabbit mAb (SDT-2351-40)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MUC5B |
| Synonyms | Mucin-5B; MUC-5B; Cervical mucin; High molecular weight salivary mucin MG1; Mucin-5 subtype B; tracheobronchial; Sublingual gland mucin; MUC5 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | Q9HC84 |
| Clone Number | SDT-2351-40 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu |
| IF | 1:500 | Hu |
Background
MUC5B, encoded on chromosome 11p15.5, is a 5,700-amino-acid gel-forming mucin produced chiefly by mucosal epithelial cells in the respiratory tract, gastrointestinal tract, cervix, and salivary glands; it polymerizes via N-terminal von Willebrand factor-like D1-D2-D′-D3 domains that disulfide-link into massive, highly glycosylated (O-linked) linear chains whose extensive carbohydrate content (up to 80 % of mass) endows viscoelastic, lubricating, and antimicrobial barrier properties essential for mucociliary clearance and host defense.
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Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-MUC5B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-MUC5B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human colon. Anti- MUC5B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows negative staining in paraffin-embedded human colon. Anti- MUC5B antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
