WB result of MTCO1 Recombinant Rabbit mAb
Primary antibody: MTCO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 37 kDa
MTCO1 Recombinant Rabbit mAb (S-2205-28)
MTCO1 Recombinant Rabbit mAb (S-2205-28)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MTCO1 |
| Synonyms | Cytochrome c oxidase subunit 1; Cytochrome c oxidase polypeptide I; COI; COXI; MT-CO1 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | P00395 |
| Clone Number | S-2205-28 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Most species |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
MTCO1 (also called COX1, COI, or MT-CO1) is the mitochondrially encoded cytochrome c oxidase subunit I, a 57 kDa, 513-amino-acid integral membrane protein that forms the catalytic core of respiratory Complex IV (cytochrome c oxidase), the terminal enzyme of the mitochondrial electron transport chain that reduces molecular oxygen to water and pumps protons to drive ATP synthesis . Located on the heavy strand of mtDNA from nucleotide 5904–7444, MTCO1 is one of only three mtDNA-encoded subunits (together with MTCO2 and MTCO3) among the 13 that make up mammalian Complex IV . Mutations or altered expression of MTCO1 have been linked to a spectrum of disorders including Leber’s hereditary optic neuropathy, Complex IV deficiency, sideroblastic anemia, sensorineural deafness, colorectal cancer, and recurrent myoglobinuria, and its levels are modulated by regulators of mitochondrial biogenesis such as PGC-1α .
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Picture
Western Blot
WB result of MTCO1 Recombinant Rabbit mAb
Primary antibody: MTCO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 35 kDa
WB result of MTCO1 Recombinant Rabbit mAb
Primary antibody: MTCO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat liver lysate 20 µg
Lane 4: rat kidney lysate 20 µg
Lane 5: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 35 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-MTCO1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-MTCO1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-MTCO1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-MTCO1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-MTCO1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-MTCO1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
