WB result of Mrp4 Recombinant Rabbit mAb
Primary antibody: Mrp4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled A549 whole cell lysate 20 µg
Lane 2: unboiled LNCaP whole cell lysate 20 µg
Lane 3: unboiled SW620 whole cell lysate 20 µg
Lane 4: unboiled HT-29 whole cell lysate 20 µg
Lane 5: unboiled U-2 OS whole cell lysate 20 µg
Lane 6: unboiled OVCAR-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 150 kDa
Observed MW: 170-280 kDa
Mrp4 Recombinant Rabbit mAb (S-2305-17)
Mrp4 Recombinant Rabbit mAb (S-2305-17)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Mrp4 |
| Synonyms | ATP-binding cassette sub-family C member 4; MRP/cMOAT-related ABC transporter; Multi-specific organic anion transporter B (MOAT-B); Multidrug resistance-associated protein 4; MOATB; MRP4; ABCC4 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | O15439 |
| Clone Number | S-2305-17 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms |
| IHC-P | 1:1000 | Hu |
Background
Mrp4 protein, also known as multidrug resistance protein 4, is a member of the ATP - binding cassette (ABC) transporter family. It is an integral membrane protein that plays a crucial role in the efflux of various substances out of cells. Mrp4 can transport a wide range of substrates, including organic anions, nucleoside - derived metabolites, and certain drugs. This protein is expressed in multiple tissues such as the liver, kidneys, and intestines. Its function is important for maintaining cellular homeostasis by preventing the accumulation of potentially toxic substances within cells. Additionally, Mrp4 has implications in the pharmacokinetics of drugs, as it can influence the absorption, distribution, metabolism, and excretion of certain medications. In the context of disease, alterations in Mrp4 expression or function have been associated with drug resistance in cancer cells and may also be involved in the pathogenesis of some renal disorders.
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Western Blot
WB result of Mrp4 Recombinant Rabbit mAb
Primary antibody: Mrp4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled NIH/3T3 whole cell lysate 20 µg
Lane 2: unboiled J774A.1 whole cell lysate 20 µg
Low expression control: unboiled NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 150 kDa
Observed MW: 170-250 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-Mrp4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-Mrp4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Mrp4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-Mrp4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
