WB result of Merlin Recombinant Rabbit mAb
Primary antibody: Merlin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 70 kDa
Merlin Recombinant Rabbit mAb (S-2331-22)
Merlin Recombinant Rabbit mAb (S-2331-22)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Merlin |
| Synonyms | Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin; SCH; NF2 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus, Cell projection |
| Accession | P35240 |
| Clone Number | S-2331-22 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, PC-3, MCF7, HepG2, MEF, mouse brain, rat brain |
| Predicted Reactivity | Bb |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt, AfGrMk |
Background
Merlin protein, also known as neurofibromin 2 (NF2), is a crucial tumor suppressor protein encoded by the NF2 gene. It plays a vital role in regulating cell proliferation, differentiation, and apoptosis. Merlin acts as a negative regulator of the Hippo signaling pathway, which is essential for controlling organ size and cell fate determination. When functioning normally, Merlin helps to inhibit the overgrowth of cells and prevents the formation of tumors. Mutations in the NF2 gene that lead to a loss of functional Merlin protein are associated with the development of various types of cancer, including schwannomas, meningiomas, and mesotheliomas. In addition to its role in cancer, Merlin is also involved in the regulation of cell adhesion and the maintenance of cellular architecture. Ongoing research is focused on understanding the detailed mechanisms of Merlin's action and exploring potential therapeutic strategies targeting the Merlin-Hippo pathway for cancer treatment.
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Western Blot
WB result of Merlin Recombinant Rabbit mAb
Primary antibody: Merlin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MEF whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 70 kDa
WB result of Merlin Recombinant Rabbit mAb
Primary antibody: Merlin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 70 kDa
WB result of Merlin Recombinant Rabbit mAb
Primary antibody: Merlin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 70 kDa
